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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 18, Iss. 7 — Jul. 1, 2001
  • pp: 1523–1530

Analytical solutions for time-resolved fluorescence lifetime imaging in a turbid medium such as tissue

David Hattery, Victor Chernomordik, Murray Loew, Israel Gannot, and Amir Gandjbakhche  »View Author Affiliations

JOSA A, Vol. 18, Issue 7, pp. 1523-1530 (2001)

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An analytical solution is developed to quantify a site-specific fluorophore lifetime perturbation that occurs, for example, when the local metabolic status is different from that of surrounding tissue. This solution may be used when fluorophores are distributed throughout a highly turbid media and the site of interest is embedded many mean scattering distances from the source and the detector. The perturbation in lifetime is differentiated from photon transit delays by random walk theory. This analytical solution requires a priori knowledge of the tissue-scattering and absorption properties at the excitation and emission wavelengths that may be obtained from concurrent time-resolved reflection measurements. Additionally, the solution has been compared with the exact, numerically solved solution. Thus the presented solution forms the basis for practical lifetime imaging in turbid media such as tissue.

© 2001 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.5280) Medical optics and biotechnology : Photon migration
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

Original Manuscript: June 7, 2000
Revised Manuscript: January 8, 2001
Manuscript Accepted: January 8, 2001
Published: July 1, 2001

David Hattery, Victor Chernomordik, Murray Loew, Israel Gannot, and Amir Gandjbakhche, "Analytical solutions for time-resolved fluorescence lifetime imaging in a turbid medium such as tissue," J. Opt. Soc. Am. A 18, 1523-1530 (2001)

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