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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 18, Iss. 8 — Aug. 1, 2001
  • pp: 2002–2008

Three-dimensional coherent transfer function for reflection confocal microscopy in the presence of refractive-index mismatch

Min Gu, Daniel Day, Osamu Nakamura, and Satoshi Kawata  »View Author Affiliations


JOSA A, Vol. 18, Issue 8, pp. 2002-2008 (2001)
http://dx.doi.org/10.1364/JOSAA.18.002002


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Abstract

The three-dimensional (3-D) coherent transfer function for reflection confocal microscopy of high-numerical-aperture objectives is derived and calculated in the presence of refractive-index mismatch when a laser beam is focused into a medium of refractive index different from its immersion medium. This aberrated coherent transfer function is then used to estimate the readout efficiency of 3-D data bits recorded in a thick medium. It is shown that the readout efficiency of confocal microscopy for 3-D bit data storage is decreased with the focal depth of an objective in a recording medium. However, a high readout efficiency can be maintained if the tube length of a reading objective is linearly altered to compensate for the spherical aberration caused by the refractive-index mismatch.

© 2001 Optical Society of America

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: September 26, 2000
Revised Manuscript: February 6, 2001
Manuscript Accepted: February 6, 2001
Published: August 1, 2001

Citation
Min Gu, Daniel Day, Osamu Nakamura, and Satoshi Kawata, "Three-dimensional coherent transfer function for reflection confocal microscopy in the presence of refractive-index mismatch," J. Opt. Soc. Am. A 18, 2002-2008 (2001)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-18-8-2002


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