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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 19, Iss. 10 — Oct. 1, 2002
  • pp: 2112–2120

New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy

Martin J. Booth, Mark A. A. Neil, and Tony Wilson  »View Author Affiliations


JOSA A, Vol. 19, Issue 10, pp. 2112-2120 (2002)
http://dx.doi.org/10.1364/JOSAA.19.002112


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Abstract

Confocal and multiphoton microscopes are particularly sensitive to specimen- or system-induced aberrations, which result in decreased resolution and signal-to-noise ratio. The inclusion of an adaptive optics correction system could help overcome this limitation and restore diffraction-limited performance, but such a system requires a suitable method of wave-front measurement. By extending the concept of a modal wave-front sensor previously described by Neil [J. Opt. Soc. Am. A 17, 1098–1107 (2000)], we present a new sensor capable of measuring directly the Zernike aberration modes introduced by a specimen. This modal sensor is particularly suited to applications in three-dimensional microscopy because of its inherent axial selectivity; only those wave fronts originating in the focal region contribute to the measured signal. Four wave-front sensor configurations are presented and their input response is characterized. Sensitivity matrices and axial responses are presented.

© 2002 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.1790) Medical optics and biotechnology : Confocal microscopy

History
Original Manuscript: June 18, 2001
Revised Manuscript: March 28, 2002
Manuscript Accepted: May 30, 2002
Published: October 1, 2002

Citation
Martin J. Booth, Mark A. A. Neil, and Tony Wilson, "New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy," J. Opt. Soc. Am. A 19, 2112-2120 (2002)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-19-10-2112


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References

  1. T. Wilson, C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, London, 1984).
  2. T. Wilson, ed., Confocal Microscopy (Academic, London, 1990).
  3. W. Denk, J. H. Strickler, W. W. Webb, “Two-photon laser scanning confocal microscopy,” Science 248, 73–76 (1990). [CrossRef] [PubMed]
  4. M. J. Booth, M. A. A. Neil, T. Wilson, “Aberration correction for confocal imaging in refractive index mismatched media,” J. Microsc. 192, 90–98 (1998). [CrossRef]
  5. J. W. Hardy, Adaptive Optics for Astronomical Telescopes (Oxford U. Press, Oxford, UK, 1998).
  6. R. K. Tyson, Principles of Adaptive Optics (Academic, London, 1991).
  7. M. A. A. Neil, M. J. Booth, T. Wilson, “New modal wave-front sensor: a theoretical analysis,” J. Opt. Soc. Am. A 17, 1098–1107 (2000). [CrossRef]
  8. M. A. A. Neil, M. J. Booth, T. Wilson, “Closed-loop aberration correction using a modal Zernike wave-front sensor,” Opt. Lett. 25, 1083–1085 (2000). [CrossRef]
  9. M. A. A. Neil, R. Juskaitis, M. J. Booth, T. Wilson, T. Tanaka, S. Kawata, “Adaptive aberration correction in a two-photon microscope,” J. Microsc. 200, 105–108 (2000). [CrossRef] [PubMed]
  10. J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, London, 1996).
  11. Min Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (World Scientific, Singapore, 1996).
  12. M. J. Booth, M. A. A. Neil, R. Juskaitis, T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Natl. Acad. Sci. USA 99, 5788–5792 (2002). [CrossRef] [PubMed]

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