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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 19, Iss. 10 — Oct. 1, 2002
  • pp: 2112–2120

New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy

Martin J. Booth, Mark A. A. Neil, and Tony Wilson  »View Author Affiliations


JOSA A, Vol. 19, Issue 10, pp. 2112-2120 (2002)
http://dx.doi.org/10.1364/JOSAA.19.002112


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Abstract

Confocal and multiphoton microscopes are particularly sensitive to specimen- or system-induced aberrations, which result in decreased resolution and signal-to-noise ratio. The inclusion of an adaptive optics correction system could help overcome this limitation and restore diffraction-limited performance, but such a system requires a suitable method of wave-front measurement. By extending the concept of a modal wave-front sensor previously described by Neil [J. Opt. Soc. Am. A 17, 1098–1107 (2000)], we present a new sensor capable of measuring directly the Zernike aberration modes introduced by a specimen. This modal sensor is particularly suited to applications in three-dimensional microscopy because of its inherent axial selectivity; only those wave fronts originating in the focal region contribute to the measured signal. Four wave-front sensor configurations are presented and their input response is characterized. Sensitivity matrices and axial responses are presented.

© 2002 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.1790) Medical optics and biotechnology : Confocal microscopy

History
Original Manuscript: June 18, 2001
Revised Manuscript: March 28, 2002
Manuscript Accepted: May 30, 2002
Published: October 1, 2002

Citation
Martin J. Booth, Mark A. A. Neil, and Tony Wilson, "New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy," J. Opt. Soc. Am. A 19, 2112-2120 (2002)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-19-10-2112

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