OSA's Digital Library

Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 19, Iss. 7 — Jul. 1, 2002
  • pp: 1406–1416

Effects of source coherence and aperture array geometry on optical sectioning strength in direct-view microscopy

Cian M. Taylor and Eithne M. McCabe  »View Author Affiliations


JOSA A, Vol. 19, Issue 7, pp. 1406-1416 (2002)
http://dx.doi.org/10.1364/JOSAA.19.001406


View Full Text Article

Acrobat PDF (430 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Laser sources offer a possible solution to the problem of low light throughput in direct-view microscopes (DVMs). However, coherent source DVMs have been shown to suffer from problems such as increased sidelobes in the depth response because of coherent cross talk between neighboring apertures. We explore theoretically how source coherence affects the depth responses of DVMs by employing various aperture spacings and number of apertures. We show that, contrary to expectation, closely spaced apertures can result in decreased full width at half-maximum of the depth response curve. We explain this as an effect of destructive interference when cross talk between neighboring apertures occurs. Using apertures arranged in a square grid as an example, we move on to show that the use of aperture arrays that consist of regularly arranged apertures can accentuate the problematic sidelobes of the depth response. We show that arranging pinholes in a rectangular grid rather than a square grid can improve the optical sectioning strength significantly. Finally, by examination of the depth responses corresponding to the infinite-pinhole-array limit, we make some general statements about source coherence and the characteristics of arrays that are likely to perform well.

© 2002 Optical Society of America

OCIS Codes
(030.1670) Coherence and statistical optics : Coherent optical effects
(180.1790) Microscopy : Confocal microscopy

Citation
Cian M. Taylor and Eithne M. McCabe, "Effects of source coherence and aperture array geometry on optical sectioning strength in direct-view microscopy," J. Opt. Soc. Am. A 19, 1406-1416 (2002)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-19-7-1406

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited