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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 21, Iss. 12 — Dec. 1, 2004
  • pp: 2257–2268

Absorption of the eye lens and macular pigment derived from the reflectance of cone photoreceptors

Niels P. A. Zagers and Dirk van Norren  »View Author Affiliations

JOSA A, Vol. 21, Issue 12, pp. 2257-2268 (2004)

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We measured the amplitude of the directional component of the bleached fundus reflectance, the so-called optical Stiles–Crawford effect, as a function of wavelength. The directional reflectance originates from within the outer segments of the photoreceptors. Thus only two anterior absorbers are of importance: macular pigment and the crystalline lens. Analysis of spectra obtained in pseudophakes established that the cone photoreceptors act as spectrally neutral reflectors. The reflectance spectra, expressed in density units, resembled the macular pigment density spectrum. Studying age effects in the lens of normal subjects resulted in a description of the optical density of the lens in terms of a “young” and an “aged” template. The young template represents the pigment O-β-glucoside of 3-hydroxykynurenine, which dominates the light absorption in young eyes and decreases with age. The aged template represents the pigments accumulating in the lens with age. The total optical density increased with age, but it was lower in the wavelength region 500–650 nm than was previously assumed on the basis of psychophysical studies. Analysis of the spectra also provided precise individual estimates of the optical density of macular pigment. Finally, we observed a decrease in the photoreceptor reflectivity with age, possibly reflecting a degradation of the photoreceptors.

© 2004 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(330.4300) Vision, color, and visual optics : Vision system - noninvasive assessment
(330.4460) Vision, color, and visual optics : Ophthalmic optics and devices
(330.5310) Vision, color, and visual optics : Vision - photoreceptors

Original Manuscript: October 2, 2003
Revised Manuscript: March 18, 2004
Manuscript Accepted: March 18, 2004
Published: December 1, 2004

Niels P. A. Zagers and Dirk van Norren, "Absorption of the eye lens and macular pigment derived from the reflectance of cone photoreceptors," J. Opt. Soc. Am. A 21, 2257-2268 (2004)

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