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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 21, Iss. 9 — Sep. 1, 2004
  • pp: 1714–1723

Vectorial, high-numerical-aperture study of phase-contrast microscopes

Peter R. T. Munro and Peter Török  »View Author Affiliations


JOSA A, Vol. 21, Issue 9, pp. 1714-1723 (2004)
http://dx.doi.org/10.1364/JOSAA.21.001714


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Abstract

We describe, using a high-numerical-aperture vectorial model, the image formation of phase-contrast microscopes. In particular, imaging of a weak phase object is considered. We show that, partly owing to the fact that phase-contrast microscopes are interference microscopes, their image formation is fundamentally different from that of conventional transmission optical microscopes. Our detailed analysis reveals a number of yet undocumented properties of these microscopes, including that depending on the given configuration, they can exhibit an improved lateral resolution when larger detectors are used in comparison with that obtained for a small detector size. We present numerical examples to explain this phenomenon and discuss our analysis in detail.

© 2004 Optical Society of America

OCIS Codes
(120.5060) Instrumentation, measurement, and metrology : Phase modulation
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(260.3160) Physical optics : Interference

History
Original Manuscript: December 4, 2003
Manuscript Accepted: April 8, 2004
Published: September 1, 2004

Citation
Peter R. T. Munro and Peter Török, "Vectorial, high-numerical-aperture study of phase-contrast microscopes," J. Opt. Soc. Am. A 21, 1714-1723 (2004)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-21-9-1714

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