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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 22, Iss. 5 — May. 1, 2005
  • pp: 892–898

Scanning holographic microscopy with transverse resolution exceeding the Rayleigh limit and extended depth of focus

Guy Indebetouw, Alouahab El Maghnouji, and Richard Foster  »View Author Affiliations

JOSA A, Vol. 22, Issue 5, pp. 892-898 (2005)

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We demonstrate experimentally that the method of scanning holographic microscopy is capable of producing images reconstructed numerically from holograms recorded digitally in the time domain by scanning, with transverse and axial resolutions comparable to those of wide-field or scanning microscopy with the same objective. Furthermore, we show that it is possible to synthesize the point-spread function of scanning holographic microscopy to obtain, with the same objective, holographic reconstructions with a transverse resolution exceeding the Rayleigh limit of the objective up to a factor of 2 in the limit of low numerical aperture. These holographic reconstructions also exhibit an extended depth of focus, the extent of which is adjustable without compromising the transverse resolution.

© 2005 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(100.6640) Image processing : Superresolution
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions
(180.6900) Microscopy : Three-dimensional microscopy

Original Manuscript: September 17, 2004
Revised Manuscript: November 10, 2004
Manuscript Accepted: November 12, 2004
Published: May 1, 2005

Guy Indebetouw, Alouahab El Maghnouji, and Richard Foster, "Scanning holographic microscopy with transverse resolution exceeding the Rayleigh limit and extended depth of focus," J. Opt. Soc. Am. A 22, 892-898 (2005)

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