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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Stephen A. Burns
  • Vol. 23, Iss. 10 — Oct. 1, 2006
  • pp: 2657–2661

A posteriori quasi-sectioning of the three-dimensional reconstructions of scanning holographic microscopy

Guy Indebetouw  »View Author Affiliations

JOSA A, Vol. 23, Issue 10, pp. 2657-2661 (2006)

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A method to obtain the axial locations of objects reconstructed by scanning holographic microscopy with submicrometer accuracy is presented and demonstrated. The method combines the holographic advantage of capturing three-dimensional (3D) information in a single two-dimensional scan, with the possibility of optically sectioning the 3D holographic reconstruction a posteriori. The method is demonstrated experimentally for pointlike features (fluorescent beads), and the limitation of the method for features of arbitrary size and shape is discussed.

© 2006 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: April 12, 2006
Manuscript Accepted: May 9, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Guy Indebetouw, "A posteriori quasi-sectioning of the three-dimensional reconstructions of scanning holographic microscopy," J. Opt. Soc. Am. A 23, 2657-2661 (2006)

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