The photosynthesis, transfer, and storage of starch are the most important biogenic processes occurring in plants. In order to observe the colorless and transparent starch granules in a plant, a chemical pretreatment such as staining of the starch is currently required, which seriously damages the tissue cells in the plant. We demonstrate that nondestructive chemical analysis of starch granules in a plant can be performed by using optical second-harmonic and sum-frequency microscopy. These techniques for in vivo analysis will provide extremely useful information about saccharides in a plant and can be extended to the analysis of many other materials, from living tissue to semiconductors.
© 2006 Optical Society of America
Original Manuscript: December 16, 2005
Manuscript Accepted: January 13, 2006
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics
Yoshihiro Miyauchi, Haruyuki Sano, and Goro Mirzutani, "Selective observation of starch in a water plant using optical sum-frequency microscopy," J. Opt. Soc. Am. A 23, 1687-1690 (2006)