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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Stephen A. Burns
  • Vol. 23, Iss. 7 — Jul. 1, 2006
  • pp: 1687–1690

Selective observation of starch in a water plant using optical sum-frequency microscopy

Yoshihiro Miyauchi, Haruyuki Sano, and Goro Mirzutani  »View Author Affiliations

JOSA A, Vol. 23, Issue 7, pp. 1687-1690 (2006)

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The photosynthesis, transfer, and storage of starch are the most important biogenic processes occurring in plants. In order to observe the colorless and transparent starch granules in a plant, a chemical pretreatment such as staining of the starch is currently required, which seriously damages the tissue cells in the plant. We demonstrate that nondestructive chemical analysis of starch granules in a plant can be performed by using optical second-harmonic and sum-frequency microscopy. These techniques for in vivo analysis will provide extremely useful information about saccharides in a plant and can be extended to the analysis of many other materials, from living tissue to semiconductors.

© 2006 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(190.0190) Nonlinear optics : Nonlinear optics

ToC Category:

Original Manuscript: December 16, 2005
Manuscript Accepted: January 13, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Yoshihiro Miyauchi, Haruyuki Sano, and Goro Mirzutani, "Selective observation of starch in a water plant using optical sum-frequency microscopy," J. Opt. Soc. Am. A 23, 1687-1690 (2006)

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