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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Stephen A. Burns
  • Vol. 23, Iss. 7 — Jul. 1, 2006
  • pp: 1699–1707

Scanning holographic microscopy of three-dimensional fluorescent specimens

Guy Indebetouw and Wenwei Zhong  »View Author Affiliations

JOSA A, Vol. 23, Issue 7, pp. 1699-1707 (2006)

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We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 μ m of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed.

© 2006 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:

Original Manuscript: September 16, 2005
Revised Manuscript: January 3, 2006
Manuscript Accepted: January 6, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Guy Indebetouw and Wenwei Zhong, "Scanning holographic microscopy of three-dimensional fluorescent specimens," J. Opt. Soc. Am. A 23, 1699-1707 (2006)

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