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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 23, Iss. 7 — Jul. 1, 2006
  • pp: 1708–1717

Point-spread function synthesis in scanning holographic microscopy

Guy Indebetouw, Wenwei Zhong, and David Chamberlin-Long  »View Author Affiliations


JOSA A, Vol. 23, Issue 7, pp. 1708-1717 (2006)
http://dx.doi.org/10.1364/JOSAA.23.001708


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Abstract

Scanning holographic microscopy is a two-pupil synthesis method allowing the capture of single-sideband in-line holograms of noncoherent (e.g., fluorescent) three-dimensional specimens in a single two-dimensional scan. The flexibility offered by the two-pupil method in synthesizing unusual point-spread functions is discussed. We illustrate and compare three examples of holographic recording, using computer simulations. The first example is the classical hologram in which each object point is encoded as a spherical wave. The second example uses pupils with spherical phase distributions having opposite curvatures, leading to reconstructed images with a resolution limit that is half that of the objective. In the third example, axicon pupils are used to obtain axially sectioned images.

© 2006 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions
(110.6880) Imaging systems : Three-dimensional image acquisition

ToC Category:
Holography

History
Original Manuscript: September 16, 2005
Manuscript Accepted: December 23, 2005

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Guy Indebetouw, Wenwei Zhong, and David Chamberlin-Long, "Point-spread function synthesis in scanning holographic microscopy," J. Opt. Soc. Am. A 23, 1708-1717 (2006)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-23-7-1708

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