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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Stephen A. Burns
  • Vol. 24, Iss. 1 — Jan. 1, 2007
  • pp: 163–168

Theoretical analysis of three-dimensional imaging and recognition of micro-organisms with a single-exposure on-line holographic microscope

Adrian Stern and Bahram Javidi  »View Author Affiliations

JOSA A, Vol. 24, Issue 1, pp. 163-168 (2007)

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Single-exposure on-line (SEOL) digital holography is a recently proposed technique for monitoring, visualization, and recognition of three-dimensional (3D) objects. In contrast to traditional multiexposure on-line digital holography, it uses only one exposure, which makes it particularly suitable for imaging and recognizing moving micro-organisms. However, the cost of using only one exposure is the superposition of a conjugate image on the desired reconstructed image. The influence of the conjugate image on the visualization and recognition performance is investigated. The conditions for which the cross-talk noise induced by the conjugate image is negligible are derived. It is demonstrated that with conditions common in imaging of microscopic 3D biological objects, SEOL digital holography is highly tolerant of cross-talk noise induced by the conjugate image.

© 2006 Optical Society of America

OCIS Codes
(100.5010) Image processing : Pattern recognition
(170.0180) Medical optics and biotechnology : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 6, 2006
Revised Manuscript: June 15, 2006
Manuscript Accepted: July 31, 2006

Virtual Issues
Vol. 2, Iss. 2 Virtual Journal for Biomedical Optics

Adrian Stern and Bahram Javidi, "Theoretical analysis of three-dimensional imaging and recognition of micro-organisms with a single-exposure on-line holographic microscope," J. Opt. Soc. Am. A 24, 163-168 (2007)

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