Single-exposure on-line (SEOL) digital holography is a recently proposed technique for monitoring, visualization, and recognition of three-dimensional (3D) objects. In contrast to traditional multiexposure on-line digital holography, it uses only one exposure, which makes it particularly suitable for imaging and recognizing moving micro-organisms. However, the cost of using only one exposure is the superposition of a conjugate image on the desired reconstructed image. The influence of the conjugate image on the visualization and recognition performance is investigated. The conditions for which the cross-talk noise induced by the conjugate image is negligible are derived. It is demonstrated that with conditions common in imaging of microscopic 3D biological objects, SEOL digital holography is highly tolerant of cross-talk noise induced by the conjugate image.
© 2006 Optical Society of America
Medical Optics and Biotechnology
Original Manuscript: February 6, 2006
Revised Manuscript: June 15, 2006
Manuscript Accepted: July 31, 2006
Vol. 2, Iss. 2 Virtual Journal for Biomedical Optics
Adrian Stern and Bahram Javidi, "Theoretical analysis of three-dimensional imaging and recognition of micro-organisms with a single-exposure on-line holographic microscope," J. Opt. Soc. Am. A 24, 163-168 (2007)