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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 24, Iss. 10 — Oct. 1, 2007
  • pp: 3261–3273

Optimizing frequency-domain fluorescence lifetime sensing for high-throughput applications: photon economy and acquisition speed

Alessandro Esposito, Hans C. Gerritsen, and Fred S. Wouters  »View Author Affiliations


JOSA A, Vol. 24, Issue 10, pp. 3261-3273 (2007)
http://dx.doi.org/10.1364/JOSAA.24.003261


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Abstract

The signal-to-noise ratio of a measurement is determined by the photon economy of the detection technique and the available photons that are emitted by the sample. We investigate the efficiency of various frequency-domain lifetime detection techniques also in relation to time-domain detection. Nonlinear effects are discussed that are introduced by the use of image intensifiers and by fluorophore saturation. The efficiency of fluorescence lifetime imaging microscopy setups is connected to the speed of acquisition and thus to the imaging throughput. We report on the optimal conditions for balancing signal-to-noise ratio and acquisition speed in fluorescence lifetime sensing.

© 2007 Optical Society of America

OCIS Codes
(030.5290) Coherence and statistical optics : Photon statistics
(110.0110) Imaging systems : Imaging systems
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Coherence and Statistical Optics

History
Original Manuscript: February 2, 2007
Revised Manuscript: July 19, 2007
Manuscript Accepted: July 21, 2007
Published: September 20, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Alessandro Esposito, Hans C. Gerritsen, and Fred S. Wouters, "Optimizing frequency-domain fluorescence lifetime sensing for high-throughput applications: photon economy and acquisition speed," J. Opt. Soc. Am. A 24, 3261-3273 (2007)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-24-10-3261

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