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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 24, Iss. 5 — May. 1, 2007
  • pp: 1411–1416

Recombinant adeno-associated virus targets passenger gene expression to cones in primate retina

Katherine Mancuso, Anita E. Hendrickson, Thomas B. Connor, Jr., Matthew C. Mauck, James J. Kinsella, William W. Hauswirth, Jay Neitz, and Maureen Neitz  »View Author Affiliations


JOSA A, Vol. 24, Issue 5, pp. 1411-1416 (2007)
http://dx.doi.org/10.1364/JOSAA.24.001411


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Abstract

Recombinant adeno-associated virus (rAAV) is a promising vector for gene therapy of photoreceptor-based diseases. Previous studies have demonstrated that rAAV serotypes 2 and 5 can transduce both rod and cone photoreceptors in rodents and dogs, and it can target rods, but not cones in primates. Here we report that using a human cone-specific enhancer and promoter to regulate expression of a green fluorescent protein (GFP) reporter gene in an rAAV-5 vector successfully targeted expression of the reporter gene to primate cones, and the time course of GFP expression was able to be monitored in a living animal using the RetCam II digital imaging system.

© 2007 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(330.0330) Vision, color, and visual optics : Vision, color, and visual optics
(330.1720) Vision, color, and visual optics : Color vision
(330.5310) Vision, color, and visual optics : Vision - photoreceptors

ToC Category:
Clinical Applications of Retinal Imaging

History
Original Manuscript: September 20, 2006
Revised Manuscript: November 21, 2006
Manuscript Accepted: November 21, 2006
Published: April 11, 2007

Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Katherine Mancuso, Anita E. Hendrickson, Thomas B. Connor, Jr., Matthew C. Mauck, James J. Kinsella, William W. Hauswirth, Jay Neitz, and Maureen Neitz, "Recombinant adeno-associated virus targets passenger gene expression to cones in primate retina," J. Opt. Soc. Am. A 24, 1411-1416 (2007)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-24-5-1411


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