Digital in-line soft x-ray holography (DIXH) was used to image immobilized polystyrene and iron oxide particles and to distinguish them based on their different x-ray absorption cross sections in the vicinity of the carbon K-absorption edge. The element-specific information from the resonant DIXH images was correlated with high-resolution scanning electron microscopy (SEM) pictures. We also present DIXH images of a cell nucleus and compare the contrast obtained for nuclear components with the appearance in optical microscopy.
© 2008 Optical Society of America
Original Manuscript: August 10, 2007
Revised Manuscript: November 16, 2007
Manuscript Accepted: November 26, 2007
Published: January 25, 2008
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics
Axel Rosenhahn, Ruth Barth, Florian Staier, Todd Simpson, Silvia Mittler, Stefan Eisebitt, and Michael Grunze, "Digital in-line soft x-ray holography with element contrast," J. Opt. Soc. Am. A 25, 416-422 (2008)