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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 25, Iss. 5 — May. 1, 2008
  • pp: 1181–1189

Automated analysis of differential interference contrast microscopy images of the foveal cone mosaic

David H. Wojtas, Bing Wu, Peter K. Ahnelt, Philip J. Bones, and R. P. Millane  »View Author Affiliations


JOSA A, Vol. 25, Issue 5, pp. 1181-1189 (2008)
http://dx.doi.org/10.1364/JOSAA.25.001181


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Abstract

An algorithm is presented for processing and analysis of differential interference contrast (DIC) microscopy images of the fovea to study the cone mosaic. The algorithm automatically locates the cones and their boundaries in such images and is assessed by comparison with results from manual analysis. Additional algorithms are presented that analyze the cone positions to extract information on cone neighbor relationships as well as the short-range order and domain structure of the mosaic. The methods are applied to DIC images of the human fovea.

© 2008 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.3170) Microscopy : Interference microscopy
(330.5310) Vision, color, and visual optics : Vision - photoreceptors

ToC Category:
Image Processing

History
Original Manuscript: June 1, 2007
Revised Manuscript: March 19, 2008
Manuscript Accepted: March 19, 2008
Published: April 28, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
David H. Wojtas, Bing Wu, Peter K. Ahnelt, Philip J. Bones, and R. P. Millane, "Automated analysis of differential interference contrast microscopy images of the foveal cone mosaic," J. Opt. Soc. Am. A 25, 1181-1189 (2008)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-25-5-1181

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