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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 26, Iss. 5 — May. 1, 2009
  • pp: 1101–1108

Optical scattering depolarization in a biomedium with anisotropic biomolecules

Tsu-Wei Nee, Soe-Mie F. Nee, De-Ming Yang, and Arthur Chiou  »View Author Affiliations


JOSA A, Vol. 26, Issue 5, pp. 1101-1108 (2009)
http://dx.doi.org/10.1364/JOSAA.26.001101


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Abstract

The depolarization property of a biomedium with anisotropic biomolecule optical scattering is investigated theoretically. By using a simple ellipsoid model of a single biomolecule, the scattering fields and Mueller matrices are derived from fundamental electromagnetism theory. The biomedium is modeled as a system of uncorrelated anisotropic molecules. On the basis of a statistical model of anisotropic molecular distribution, the scattering depolarization of the biomedium is investigated. Simulated results of the molecular shape and orientation dependent single scattering depolarization D 1 and the double scattering depolarization D 2 are reported. The D 2 contribution is found to be more important for higher-density scattering media. The depolarizations of the forward single and double scattering of a model cell membrane are simulated and discussed. The fitting to a single tetra-methylrhodamine-labeled lipid molecule’s anisotropic imaging experiment has demonstrated that large depolarization arises for the membrane to which the fluorescence emitting molecule is attached. This theory can provide a simulation analysis tool for investigating the scattering polarization/depolarization effect and the photon density wave transport property of a highly scattering biomedium.

© 2009 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(290.1350) Scattering : Backscattering
(290.5840) Scattering : Scattering, molecules
(290.5825) Scattering : Scattering theory
(290.5855) Scattering : Scattering, polarization

ToC Category:
Scattering

History
Original Manuscript: January 6, 2009
Manuscript Accepted: February 10, 2009
Published: April 7, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Tsu-Wei Nee, Soe-Mie F. Nee, De-Ming Yang, and Arthur Chiou, "Optical scattering depolarization in a biomedium with anisotropic biomolecules," J. Opt. Soc. Am. A 26, 1101-1108 (2009)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-26-5-1101


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References

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