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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Franco Gori
  • Vol. 28, Iss. 10 — Oct. 1, 2011
  • pp: 2108–2114

Quantitative determination of dynamical properties using coherent spatial frequency domain imaging

Tyler B. Rice, Soren D. Konecky, Amaan Mazhar, David J. Cuccia, Anthony J. Durkin, Bernard Choi, and Bruce J. Tromberg  »View Author Affiliations

JOSA A, Vol. 28, Issue 10, pp. 2108-2114 (2011)

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Laser speckle imaging (LSI) is a fast, noninvasive method to obtain relative particle dynamics in highly light scattering media, such as biological tissue. To make quantitative measurements, we combine LSI with spatial frequency domain imaging, a technique where samples are illuminated with sinusoidal intensity patterns of light that control the characteristic path lengths of photons in the sample. We use both diffusion and radiative transport to predict the speckle contrast of coherent light remitted from turbid media. We validate our technique by measuring known Brownian diffusion coefficients ( D b ) of scattering liquid phantoms. Monte Carlo (MC) simulations of radiative transport were found to provide the most accurate contrast predictions. For polystyrene microspheres of radius 800 nm in water, the expected and fit D b using radiative transport were 6.10 E 07 and 7.10 E 07 mm 2 / s , respectively. For polystyrene microspheres of radius 1026 nm in water, the expected and fit D b were 4.7 E 07 and 5.35 mm 2 / s , respectively. For scattering particles in water–glycerin solutions, the fit fractional changes in D b with changes in viscosity were all found to be within 3% of the expected value.

© 2011 Optical Society of America

OCIS Codes
(110.6150) Imaging systems : Speckle imaging
(170.6480) Medical optics and biotechnology : Spectroscopy, speckle

ToC Category:
Imaging Systems

Original Manuscript: April 12, 2011
Revised Manuscript: July 25, 2011
Manuscript Accepted: August 17, 2011
Published: September 21, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Tyler B. Rice, Soren D. Konecky, Amaan Mazhar, David J. Cuccia, Anthony J. Durkin, Bernard Choi, and Bruce J. Tromberg, "Quantitative determination of dynamical properties using coherent spatial frequency domain imaging," J. Opt. Soc. Am. A 28, 2108-2114 (2011)

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