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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Franco Gori
  • Vol. 29, Iss. 10 — Oct. 1, 2012
  • pp: 2104–2109

Experimental validation of phase using Nomarski microscopy with an extended Fried algorithm

Scott A. Prahl, Amanda Dayton, Kyle Juedes, Erik J. Sánchez, Rafael Páez López, and Donald D. Duncan  »View Author Affiliations


JOSA A, Vol. 29, Issue 10, pp. 2104-2109 (2012)
http://dx.doi.org/10.1364/JOSAA.29.002104


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Abstract

Reconstruction of an image (or shape or wavefront) from measurements of the derivatives of the image in two orthogonal directions is a common problem. We demonstrate how a particular reconstructor, commonly referred to as the Fried algorithm, can be used with megapixel derivative images to recover the original image. Large datasets are handled by breaking the derivative images into smaller tiles, applying the Fried algorithm and stitching the tiles back together. The performance of the algorithm is demonstrated using differential interference contrast microscopy on a known test object.

© 2012 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(180.3170) Microscopy : Interference microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Microscopy

History
Original Manuscript: May 4, 2012
Revised Manuscript: August 24, 2012
Manuscript Accepted: August 25, 2012
Published: September 12, 2012

Citation
Scott A. Prahl, Amanda Dayton, Kyle Juedes, Erik J. Sánchez, Rafael Páez López, and Donald D. Duncan, "Experimental validation of phase using Nomarski microscopy with an extended Fried algorithm," J. Opt. Soc. Am. A 29, 2104-2109 (2012)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-29-10-2104

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