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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Franco Gori
  • Vol. 29, Iss. 12 — Dec. 1, 2012
  • pp: 2696–2707

Computational modeling of optical projection tomographic microscopy using the finite difference time domain method

Ryan L. Coe and Eric J. Seibel  »View Author Affiliations

JOSA A, Vol. 29, Issue 12, pp. 2696-2707 (2012)

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We present a method for modeling image formation in optical projection tomographic microscopy (OPTM) using high numerical aperture (NA) condensers and objectives. Similar to techniques used in computed tomography, OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The model is capable of simulating axial scanning of a microscope objective to produce projections, which are reconstructed using filtered backprojection. Simulation of optical scattering in transmission optical microscopy is designed to analyze all aspects of OPTM image formation, such as degree of specimen staining, refractive-index matching, and objective scanning. In this preliminary work, a set of simulations is performed to examine the effect of changing the condenser NA, objective scan range, and complex refractive index on the final reconstruction of a microshell with an outer radius of 1.5 μm and an inner radius of 0.9 μm. The model lays the groundwork for optimizing OPTM imaging parameters and triaging efforts to further improve the overall system design. As the model is expanded in the future, it will be used to simulate a more realistic cell, which could lead to even greater impact.

© 2012 Optical Society of America

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(110.2990) Imaging systems : Image formation theory
(180.6900) Microscopy : Three-dimensional microscopy
(290.0290) Scattering : Scattering
(050.1755) Diffraction and gratings : Computational electromagnetic methods

ToC Category:

Original Manuscript: September 21, 2012
Revised Manuscript: November 2, 2012
Manuscript Accepted: November 7, 2012
Published: November 30, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Ryan L. Coe and Eric J. Seibel, "Computational modeling of optical projection tomographic microscopy using the finite difference time domain method," J. Opt. Soc. Am. A 29, 2696-2707 (2012)

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