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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Franco Gori
  • Vol. 29, Iss. 7 — Jul. 1, 2012
  • pp: 1277–1287

Setup for single-particle orbit tracking: artifacts and corrections

Dominique Ernst, Stefan Hain, and Jürgen Köhler  »View Author Affiliations


JOSA A, Vol. 29, Issue 7, pp. 1277-1287 (2012)
http://dx.doi.org/10.1364/JOSAA.29.001277


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Abstract

We report on an experimental setup for single-particle orbit tracking, which allows following fluorescent nanoparticles for more than 10 min with a temporal resolution of 4 ms and a dynamic position accuracy of better than 10 nm. On a model sample—20 nm sized fluorescent polymer beads in glycerol—we will illustrate how artifacts caused by unavoidable experimental shortcomings (might) obscure the experimental result and how misinterpretations can be prevented.

© 2012 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Microscopy

History
Original Manuscript: March 16, 2012
Manuscript Accepted: March 30, 2012
Published: June 7, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Dominique Ernst, Stefan Hain, and Jürgen Köhler, "Setup for single-particle orbit tracking: artifacts and corrections," J. Opt. Soc. Am. A 29, 1277-1287 (2012)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-29-7-1277

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