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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Franco Gori
  • Vol. 29, Iss. 8 — Aug. 1, 2012
  • pp: 1606–1614

Ptychographic transmission microscopy in three dimensions using a multi-slice approach

A. M. Maiden, M. J. Humphry, and J. M. Rodenburg  »View Author Affiliations


JOSA A, Vol. 29, Issue 8, pp. 1606-1614 (2012)
http://dx.doi.org/10.1364/JOSAA.29.001606


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Abstract

Generally, methods of three-dimensional imaging such as confocal microscopy and computed tomography rely on two essentials: multiple measurements (at a range of focus positions or rotations) and a weakly scattering specimen (to avoid distortion of the focal spot in the confocal microscope or to satisfy the projection approximation in tomography). Here we show that an alternative form of multi-measurement imaging, ptychography, can be extended to three dimensions and can successfully recover images in the presence of multiple scattering and when the projection approximation is not applicable. We demonstrate our technique experimentally using visible light, where it has applications in imaging thick samples such as biological tissues; however the results also have important implications for x ray and electron imaging.

© 2012 Optical Society of America

OCIS Codes
(100.3190) Image processing : Inverse problems
(100.5070) Image processing : Phase retrieval
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Image Processing

History
Original Manuscript: March 6, 2012
Revised Manuscript: June 15, 2012
Manuscript Accepted: June 15, 2012
Published: July 20, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

Citation
A. M. Maiden, M. J. Humphry, and J. M. Rodenburg, "Ptychographic transmission microscopy in three dimensions using a multi-slice approach," J. Opt. Soc. Am. A 29, 1606-1614 (2012)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-29-8-1606

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