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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Franco Gori
  • Vol. 30, Iss. 10 — Oct. 1, 2013
  • pp: 1980–1987

Phase diversity for three-dimensional imaging

Peter Kner  »View Author Affiliations

JOSA A, Vol. 30, Issue 10, pp. 1980-1987 (2013)

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Phase diversity (PD) is a powerful technique for estimating wavefront aberrations from two-dimensional images of extended scenes. PD can work with extended incoherent images and, in an adaptive optics system, does not need extra hardware in addition to the deformable mirror. For these reasons, PD should be well suited to aberration measurement in microscopy applications. But, in biological widefield microscopy, the objects being imaged are frequently three-dimensional, and the images contain out-of-focus light. In this paper, we introduce multiplane PD and show that PD can be extended to widefield imaging of three-dimensional objects. This should be particularly useful in the field of biological fluorescence microscopy where the objects are very light sensitive and the aberrations cannot easily be determined.

© 2013 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(100.6890) Image processing : Three-dimensional image processing
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: June 12, 2013
Revised Manuscript: August 5, 2013
Manuscript Accepted: August 18, 2013
Published: September 12, 2013

Peter Kner, "Phase diversity for three-dimensional imaging," J. Opt. Soc. Am. A 30, 1980-1987 (2013)

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