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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Franco Gori
  • Vol. 30, Iss. 12 — Dec. 1, 2013
  • pp: 2595–2604

Automated segmentation of retinal pigment epithelium cells in fluorescence adaptive optics images

Piero Rangel-Fonseca, Armando Gómez-Vieyra, Daniel Malacara-Hernández, Mario C. Wilson, David R. Williams, and Ethan A. Rossi  »View Author Affiliations


JOSA A, Vol. 30, Issue 12, pp. 2595-2604 (2013)
http://dx.doi.org/10.1364/JOSAA.30.002595


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Abstract

Adaptive optics (AO) imaging methods allow the histological characteristics of retinal cell mosaics, such as photoreceptors and retinal pigment epithelium (RPE) cells, to be studied in vivo. The high-resolution images obtained with ophthalmic AO imaging devices are rich with information that is difficult and/or tedious to quantify using manual methods. Thus, robust, automated analysis tools that can provide reproducible quantitative information about the cellular mosaics under examination are required. Automated algorithms have been developed to detect the position of individual photoreceptor cells; however, most of these methods are not well suited for characterizing the RPE mosaic. We have developed an algorithm for RPE cell segmentation and show its performance here on simulated and real fluorescence AO images of the RPE mosaic. Algorithm performance was compared to manual cell identification and yielded better than 91% correspondence. This method can be used to segment RPE cells for morphometric analysis of the RPE mosaic and speed the analysis of both healthy and diseased RPE mosaics.

© 2013 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(100.2960) Image processing : Image analysis
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(330.4300) Vision, color, and visual optics : Vision system - noninvasive assessment
(100.4995) Image processing : Pattern recognition, metrics
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

History
Original Manuscript: September 5, 2013
Revised Manuscript: November 4, 2013
Manuscript Accepted: November 5, 2013
Published: November 21, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Piero Rangel-Fonseca, Armando Gómez-Vieyra, Daniel Malacara-Hernández, Mario C. Wilson, David R. Williams, and Ethan A. Rossi, "Automated segmentation of retinal pigment epithelium cells in fluorescence adaptive optics images," J. Opt. Soc. Am. A 30, 2595-2604 (2013)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-30-12-2595

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