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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Franco Gori
  • Vol. 30, Iss. 4 — Apr. 1, 2013
  • pp: 769–782

Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation

Michael Shribak  »View Author Affiliations


JOSA A, Vol. 30, Issue 4, pp. 769-782 (2013)
http://dx.doi.org/10.1364/JOSAA.30.000769


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Abstract

We describe a quantitative orientation-independent differential interference contrast (DIC) microscope, which allows bias retardation to be modulated and shear directions to be switched rapidly without any mechanical movement. The shear direction is switched by a regular liquid-crystal cell sandwiched between two standard DIC prisms. Another liquid-crystal cell modulates the bias. Techniques for measuring parameters of DIC prisms and calibrating the bias are shown. Two sets of raw DIC images with the orthogonal shear directions are captured within 1 s. Then the quantitative image of optical path gradient distribution within a thin optical section is computed. The gradient data are used to obtain a quantitative distribution of the optical path, which represents the refractive index gradient or height distribution. Computing enhanced regular DIC images with any desired shear direction is also possible.

© 2013 Optical Society of America

OCIS Codes
(100.2980) Image processing : Image enhancement
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.3940) Instrumentation, measurement, and metrology : Metrology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(180.0180) Microscopy : Microscopy

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: December 21, 2012
Revised Manuscript: February 20, 2013
Manuscript Accepted: February 20, 2013
Published: March 27, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Michael Shribak, "Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation," J. Opt. Soc. Am. A 30, 769-782 (2013)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-30-4-769

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