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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Franco Gori
  • Vol. 31, Iss. 5 — May. 1, 2014
  • pp: 968–980

Image formation of thick three-dimensional objects in differential-interference-contrast microscopy

Sigal Trattner, Eugene Kashdan, Micha Feigin, and Nir Sochen  »View Author Affiliations


JOSA A, Vol. 31, Issue 5, pp. 968-980 (2014)
http://dx.doi.org/10.1364/JOSAA.31.000968


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Abstract

The differential-interference-contrast (DIC) microscope is of widespread use in life sciences as it enables noninvasive visualization of transparent objects. The goal of this work is to model the image formation process of thick three-dimensional objects in DIC microscopy. The model is based on the principles of electromagnetic wave propagation and scattering. It simulates light propagation through the components of the DIC microscope to the image plane using a combined geometrical and physical optics approach and replicates the DIC image of the illuminated object. The model is evaluated by comparing simulated images of three-dimensional spherical objects with the recorded images of polystyrene microspheres. Our computer simulations confirm that the model captures the major DIC image characteristics of the simulated object, and it is sensitive to the defocusing effects.

© 2014 Optical Society of America

OCIS Codes
(110.2990) Imaging systems : Image formation theory
(180.0180) Microscopy : Microscopy
(290.0290) Scattering : Scattering

ToC Category:
Microscopy

History
Original Manuscript: November 4, 2013
Revised Manuscript: January 27, 2014
Manuscript Accepted: February 26, 2014
Published: April 9, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Sigal Trattner, Eugene Kashdan, Micha Feigin, and Nir Sochen, "Image formation of thick three-dimensional objects in differential-interference-contrast microscopy," J. Opt. Soc. Am. A 31, 968-980 (2014)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-31-5-968


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