A new principle of tomographic optical microscope imaging has been developed that uses a computerized reconstruction algorithm and a transmission optical microscope. A conventional transmission microscope with a rotationally oblique illumination system provides projections of a thick specimen in various directions within the numerical aperture of the objective lens. The images obtained are combined to reconstruct a three-dimensional distribution of the sample by inverting the imaging system in a computer. The three-dimensional optical transfer function of this projection system is analyzed, and it is found that this system is strictly angularly band limited. For improving the spatial resolution we utilize a priori knowledge of the spatial extent of the object as the support constraint. Experimental results are presented to demonstrate the tomographic imaging capability of this principle.
© 1987 Optical Society of America
Original Manuscript: May 26, 1986
Manuscript Accepted: September 23, 1986
Published: January 1, 1987
S. Kawata, O. Nakamura, and S. Minami, "Optical microscope tomography. I. Support constraint," J. Opt. Soc. Am. A 4, 292-297 (1987)