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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 4, Iss. 1 — Jan. 1, 1987
  • pp: 292–297

Optical microscope tomography. I. Support constraint

S. Kawata, O. Nakamura, and S. Minami  »View Author Affiliations


JOSA A, Vol. 4, Issue 1, pp. 292-297 (1987)
http://dx.doi.org/10.1364/JOSAA.4.000292


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Abstract

A new principle of tomographic optical microscope imaging has been developed that uses a computerized reconstruction algorithm and a transmission optical microscope. A conventional transmission microscope with a rotationally oblique illumination system provides projections of a thick specimen in various directions within the numerical aperture of the objective lens. The images obtained are combined to reconstruct a three-dimensional distribution of the sample by inverting the imaging system in a computer. The three-dimensional optical transfer function of this projection system is analyzed, and it is found that this system is strictly angularly band limited. For improving the spatial resolution we utilize a priori knowledge of the spatial extent of the object as the support constraint. Experimental results are presented to demonstrate the tomographic imaging capability of this principle.

© 1987 Optical Society of America

History
Original Manuscript: May 26, 1986
Manuscript Accepted: September 23, 1986
Published: January 1, 1987

Citation
S. Kawata, O. Nakamura, and S. Minami, "Optical microscope tomography. I. Support constraint," J. Opt. Soc. Am. A 4, 292-297 (1987)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-4-1-292

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