OSA's Digital Library

Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 8, Iss. 3 — Mar. 1, 1991
  • pp: 511–519

Methods of improving the images in phase-contrast microscopy: theory and computer simulation

Iku Nemoto and Akira Takahashi  »View Author Affiliations


JOSA A, Vol. 8, Issue 3, pp. 511-519 (1991)
http://dx.doi.org/10.1364/JOSAA.8.000511


View Full Text Article

Enhanced HTML    Acrobat PDF (2040 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Three methods for improving the image of phase-contrast microscopy are proposed. The first two methods subtract, from the ordinary phase-contrast image, the image obtained through an objective with different pupil functions from that of the ordinary objective. These methods can reduce the distortion due to nonlinearity to less than 1/10 of the ordinary phase-contrast image. The third method simply uses a brighter objective lens along with a television camera with offset adjustment. The brighter lens results in higher sensitivity and less distortion. Theory, computer simulation, and a few experimental results are presented.

© 1991 Optical Society of America

History
Original Manuscript: March 6, 1990
Manuscript Accepted: October 17, 1990
Published: March 1, 1991

Citation
Iku Nemoto and Akira Takahashi, "Methods of improving the images in phase-contrast microscopy: theory and computer simulation," J. Opt. Soc. Am. A 8, 511-519 (1991)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-8-3-511


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. R. D. Allen, J. L. Travis, N. S. Allen, H. Yilmaz, “Video-enhanced contrast polarization (AVEC-POL) microscopy,” Cell Motil. 1, 275–289 (1981). [CrossRef]
  2. N. Streibl, “Three-dimensional imaging by a microscope,” J. Opt. Soc. Am. A 2, 121–127 (1985). [CrossRef]
  3. I. Nemoto, “Three-dimensional imaging in microscopy as an extension of the theory of two-dimensional imaging,” J. Opt. Soc. Am. A 5, 1848–1851 (1988). [CrossRef]
  4. H. H. Hopkins, “The frequency response of a defocused optical system,” Proc. R. Soc. London Ser. A 231, 91–103 (1957).
  5. M. Born, E. Wolf, Principles of Optics (Pergamon, Oxford, 1980).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited