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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 8, Iss. 3 — Mar. 1, 1991
  • pp: 511–519

Methods of improving the images in phase-contrast microscopy: theory and computer simulation

Iku Nemoto and Akira Takahashi  »View Author Affiliations


JOSA A, Vol. 8, Issue 3, pp. 511-519 (1991)
http://dx.doi.org/10.1364/JOSAA.8.000511


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Abstract

Three methods for improving the image of phase-contrast microscopy are proposed. The first two methods subtract, from the ordinary phase-contrast image, the image obtained through an objective with different pupil functions from that of the ordinary objective. These methods can reduce the distortion due to nonlinearity to less than 1/10 of the ordinary phase-contrast image. The third method simply uses a brighter objective lens along with a television camera with offset adjustment. The brighter lens results in higher sensitivity and less distortion. Theory, computer simulation, and a few experimental results are presented.

© 1991 Optical Society of America

History
Original Manuscript: March 6, 1990
Manuscript Accepted: October 17, 1990
Published: March 1, 1991

Citation
Iku Nemoto and Akira Takahashi, "Methods of improving the images in phase-contrast microscopy: theory and computer simulation," J. Opt. Soc. Am. A 8, 511-519 (1991)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-8-3-511

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