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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 9, Iss. 1 — Jan. 1, 1992
  • pp: 151–153

Confocal fluorescent microscopy with a finite-sized circular detector

Min Gu and C. J. R. Sheppard  »View Author Affiliations


JOSA A, Vol. 9, Issue 1, pp. 151-153 (1992)
http://dx.doi.org/10.1364/JOSAA.9.000151


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Abstract

For a confocal fluorescent microscope with a finite-sized circular detector, the three-dimensional optical transfer function (OTF) for a thick object has been developed without the use of Stockseth’s approximation. The results show that the OTF has negative values when the radius of the detector exceeds certain magnitudes. The two-dimensional OTF derived from the three-dimensional OTF is also given.

© 1992 Optical Society of America

History
Original Manuscript: September 7, 1990
Revised Manuscript: May 15, 1991
Manuscript Accepted: August 7, 1991
Published: January 1, 1992

Citation
Min Gu and C. J. R. Sheppard, "Confocal fluorescent microscopy with a finite-sized circular detector," J. Opt. Soc. Am. A 9, 151-153 (1992)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-9-1-151

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