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Journal of the Optical Society of America B

Journal of the Optical Society of America B

| OPTICAL PHYSICS

  • Vol. 13, Iss. 3 — Mar. 1, 1996
  • pp: 481–491

Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm

Chris Xu and Watt W. Webb  »View Author Affiliations


JOSA B, Vol. 13, Issue 3, pp. 481-491 (1996)
http://dx.doi.org/10.1364/JOSAB.13.000481


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Abstract

Measurements of two-photon fluorescence excitation (TPE) spectra are presented for 11 common molecular fluorophores in the excitation wavelength range 690 nm < λ < 1050 nm. Results of excitation by ˜100-fs pulses of a mode-locked Ti:sapphire laser are corroborated by single-mode cw Ti:sapphire excitation data in the range 710 nm < λ < 840 nm. Absolute values of the TPE cross section for Rhodamine B and Fluorescein are obtained by comparison with one-photon-excited fluorescence, assuming equal emission quantum efficiencies. TPE action cross sections for the other nine fluorophores are also determined. No differences between one-photon- and two-photon-excited fluorescence emission spectra are found. TPE emission spectra are independent of excitation wavelength. With both pulsed and cw excitation the fluorescence emission intensities are strictly proportional to the square of the excitation intensity to within ±4% for excitation intensities sufficiently below excited-state saturation.

© 1996 Optical Society of America

Citation
Chris Xu and Watt W. Webb, "Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm," J. Opt. Soc. Am. B 13, 481-491 (1996)
http://www.opticsinfobase.org/josab/abstract.cfm?URI=josab-13-3-481


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References

  1. M. Göppert-Mayer, "Über Elementarakte mit zwei Quanten-sprüngen," Ann. Phys. 9, 273–295 (1931).
  2. W. Kaiser and C. G. B. Garrett, "Two-photon excitation in CaF2:Eu2+," Phys. Rev. Lett. 7, 229–231 (1961).
  3. A. J. Twarowski and D. S. Kliger, "Multiphoton absorption spectra using thermal blooming," Chem. Phys. 20, 259–264 (1977).
  4. M. Sheik-Bahae, A. A. Said, T. Wei, D. Hagan, and E. W. Van Stryland, "Sensitive measurement of optical nonlinearities using a single beam," IEEE J. Quantum Electron. 26, 760–769 (1990).
  5. J. P. Hermann and J. Ducuing, "Absolute measurement of two-photon cross sections," Phys. Rev. A 5, 2557–2568 (1972).
  6. R. Swofford and W. M. McClain, "The effect of spatial and temporal laser beam characteristics on two-photon absorption," Chem. Phys. Lett. 34, 455–460 (1975).
  7. R. R. Birge, "One-photon and two-photon excitation spectroscopy," in Ultrasensitive Laser Spectroscopy, D. S. Kliger, ed. (Academic, New York, 1983), pp. 109–174.
  8. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73–76 (1990).
  9. W. M. McClain and R. A. Harris, "Two-photon molecular spectroscopy in liquids and gases," in Excited States, E. C. Lim, ed. (Academic, New York, 1977), pp. 1–56.
  10. A. Fischer, C. Cremer, and E. H. K. Stelzer, "Fluorescence of coumarins and xanthenes after two-photon absorption with a pulsed titanium-sapphire laser," Appl. Opt. 34, 1989–2003 (1995).
  11. R. Loudon, The Quantum Theory of Light, 2nd ed. (Clarendon, Oxford, 1983), pp. 82–119.
  12. T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, New York, 1984), pp. 25.
  13. C. J. R. Sheppard and H. J. Matthews, "Imaging in high-aperture optical systems," J. Opt. Soc. Am. A 4, 1354–1360 (1987).
  14. J. Guild, Applied Physics, Cornell University, Ithaca, New York 14853 (personal communications, 1995). The estimated relative accuracy of the volume integration is 3%.
  15. Assuming that the sample thickness is much greater than the Raleigh length of the Gaussian beam, the appropriate form for <F(t)> is <F(t)> = ½gφη2Cδ(nπ/λ)<P(t)>2. We note that the total fluorescence generation is independent of beam waist size.
  16. J. Kafka, M. L. Watts, and J. W. Pieterse, "Picosecond and femtosecond pulses generation in a regeneratively mode-locked Ti:S laser," IEEE J. Quantum Electron. 28, 2151–2161 (1992).
  17. S. Hell, G. Reiner, C. Cremer, and E. H. K. Stelzer, "Aberrations in confocal microscopy induced by mismatches in refractive index," J. Microsc. 169, 391–405 (1993).
  18. J. N. Demas and G. A. Crosby, "The measurement of photo-luminescence quantum yields," J. Phys. Chem. 75, 991–1024 (1971).
  19. G. Grynkiewicz, M. Poenie, and R. Y. Tsien, "A new generation of Ca2+ indicators with greatly improved fluorescence properties," J. Bio. Chem. 260, 3440–3450 (1985).
  20. DAPI not bound to DNA. The fluorescence quantum efficiency of DAPI is expected to go up by 20-fold upon binding to DNA (from Molecular Probes Handbook 1992–1994, R. P. Haugland, ed., p. 222).
  21. B. Dick and G. Hohlneicher, "Importance of initial and final states as intermediate states in two-photon spectroscopy of polar molecules," J. Chem. Phys. 76, 5755–5760 (1982).
  22. D. J. Bradley, M. H. R. Hutchinson, T. M. H. Koetser, G. H. C. New, and M. S. Petty, "Interaction of picosecond laser pulses with organic molecules I: Two-photon fluorescence quenching and singlet states excitation in rhodamine dyes," Proc. R. Soc. London Ser. A 328, 97–121 (1972).
  23. J. P. Hermann and J. Ducuing, "Dispersion of the two-photon cross-section in rhodamine dyes," Opt. Commun. 6, 101–105 (1972).
  24. L. Goodman and R. P. Pava, "Two-photon spectra of aromatic molecules," Acc. Chem. Res. 17, 250–257 (1984).
  25. D. J. Bradley, M. H. R. Hutchinson, and H. Koetser, "Interaction of picosecond laser pulses with organic molecules II: Two-photon absorption cross-sections," Proc. R. Soc. Lond. A 329, 105–119 (1972).
  26. S. M. Kennedy and F. E. Lytle, "p-Bis(o-methylstyryl) benzene as a power-square sensor for two-photon absorption measurements between 537 and 694 nm," Anal. Chem. 58, 2643–2647 (1986).
  27. I. Gryczynski, J. Kusba, V. Bogdanov, and J. R. Lakowicz, "Quenching of fluorescence by light: a new method to control the excited-state lifetime and orientations of fluorophores," J. Fluoresc. 4, 103–109 (1994).
  28. C. Xu, J. Guild, and W. W. Webb, "Two-photon fluorescence excitation spectra of calcium probe indo-1," Biophys. J. 66, A161 (1994).
  29. C. Xu, J. Guild, and W. W. Webb, "Two-photon excitation cross-sections for commonly used biological fluorophores," Biophys. J. 68, A197 (1995).
  30. J. D. Kafka and T. Baer, "Prism-pair delay lines in optical pulse compression," Opt. Lett. 12, 401–403 (1987).
  31. L. F. Mollenauer, R. H. Stolen, and J. P. Gordon, "Experimental observation of picosecond pulse narrowing and solitons in optical fibers," Phys. Rev. Lett. 45, 1095–1098 (1980).
  32. Z. Bor, "Distortion of femtosecond laser pulses in lenses and lens systems," J. Mod. Opt. 35, 1907–1918 (1988).
  33. J. Guild, C. Xu, and W. W. Webb, "Measurement of group delay dispersion of high numerical aperture objectives using two-photon excited fluorescence," submitted to Appl. Opt.
  34. Two-photon transitions consist of two separate one-photon transitions.
  35. P. F. Curley, A. I. Ferguson, J. G. White, and W. B. Amos, "Application of a femtosecond self-sustaining mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy," Opt. Quantum Electron. 24, 851–859 (1992).
  36. L. Parma and N. Omenetto, "Fluorescence behavior of 7-hydroxycoumarine excited by one-photon and two-photon absorption by means of a tunable dye laser," Chem. Phys. Lett. 54, 544–546 (1978).
  37. R. D. Jones and P. R. Callis, "A power-square sensor for two-photon spectroscopy and dispersion of second-order coherence," J. Appl. Phys. 64, 4301–4305 (1988).
  38. C. Xu, J. Guild, W. W. Webb, and W. Denk, "Determination of absolute two-photon excitation cross-sections by in situ second-order autocorrelation," Opt. Lett. 21, 2372–2374 (1995).
  39. W. L. Smith, "Two-photon absorption in condensed media," in Handbook of Laser Science and Technology, J. Weber, ed. (CRC, Baco Raton, Fla., 1986), pp. 229–258.
  40. C. J. R. Sheppard and M. Gu, "Image formation in two-photon fluorescence microscopy," Optik (Stuttgart) 86, 104–106 (1990).
  41. G. S. He, G. C. Xu, P. N. Prasad, B. A. Reinhardt, J. C. Bhatt, and A. G. Dillard, "Two-photon absorption and optical-limiting properties of novel organic compounds," Opt. Lett. 20, 435–437 (1995).
  42. J. Zyss, I. Ledoux, and J. F. Nicoud, "Advances in molecular engineering for quadratic nonlinear optics," in Molecular Nonlinear Optics, J. Zyss, ed. (Academic, San Diego, Calif., 1994), pp. 130–200.

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