OSA's Digital Library

Journal of the Optical Society of America B

Journal of the Optical Society of America B

| OPTICAL PHYSICS

  • Vol. 17, Iss. 6 — Jun. 1, 2000
  • pp: 1058–1067

Accumulated photon-echo spectroscopy for stained tissue samples

K. Uchikawa, M. Sakamoto, A. Koreeda, and S. Saikan  »View Author Affiliations


JOSA B, Vol. 17, Issue 6, pp. 1058-1067 (2000)
http://dx.doi.org/10.1364/JOSAB.17.001058


View Full Text Article

Enhanced HTML    Acrobat PDF (501 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We performed phase relaxation-time measurements of the accumulated photon echo for normal and cancerous tissue samples of human liver, that were stained with several kinds of dye. Each dye interacted selectively with a specific kind of biomolecule in the tissues, for example, DNA, proteins, or lipids. The cancerous tissues showed a tendency to have shorter relaxation times than the normal tissues had. The difference of relaxation time observed between the normal and the cancerous tissues depended on the dyes that were used for staining, and the difference became most clear when the tissues were stained with a cyanine dye derivative, i.e., YO-PRO3 Iodide, which has a high selectivity for DNA. We performed the relaxation-time mapping of the accumulated photon echo (photon-echo imaging) for a liver tissue sample stained by YO-PRO3 Iodide. The photon-echo imaging was successful in showing a region of a highly differentiated hepatocellular carcinoma in the tissue sample, while fluorescence-intensity mapping could not identify the cancerous region in the same tissue sample. These results indicated a possibility that the reduction of accumulated photon-echo relaxation time observed in the cancerous tissues was caused by a change of microscopic dynamics of DNA.

© 2000 Optical Society of America

OCIS Codes
(170.6930) Medical optics and biotechnology : Tissue
(260.2510) Physical optics : Fluorescence
(270.1670) Quantum optics : Coherent optical effects
(300.6420) Spectroscopy : Spectroscopy, nonlinear
(300.6500) Spectroscopy : Spectroscopy, time-resolved

Citation
K. Uchikawa, M. Sakamoto, A. Koreeda, and S. Saikan, "Accumulated photon-echo spectroscopy for stained tissue samples," J. Opt. Soc. Am. B 17, 1058-1067 (2000)
http://www.opticsinfobase.org/josab/abstract.cfm?URI=josab-17-6-1058

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited