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Journal of the Optical Society of America B

Journal of the Optical Society of America B


  • Vol. 18, Iss. 7 — Jul. 1, 2001
  • pp: 948–953

Concurrent enhancement of imaging depth and contrast for optical coherence tomography by hyperosmotic agents

Ruikang K. Wang, Xiangqun Xu, Valery V. Tuchin, and James B. Elder  »View Author Affiliations

JOSA B, Vol. 18, Issue 7, pp. 948-953 (2001)

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The highly scattering nature of nontransparent human tissue limits the imaging depth of optical coherence tomography (OCT) to 1–2 mm. When the longer wavelength of the light source is used; the penetration depth is improved; however, the imaging contrast is decreased, largely because of reduced backscattering at the microscopic scale and reduced refractive heterogeneity of the macroscopic scale. For a more effective diagnosis using OCT, a concurrent improvement of penetration depth and imaging contrast is often needed. We report that the OCT imaging depth and contrast can be enhanced concurrently by the use of osmotic agents. Imaging depth enhancement by application of a chemical agent is not new; however, to our knowledge; imaging contrast enhancement has not been reported in the literature. Our hypothesis is that the latter is due to localized dehydration. We demonstrate experimentally, by examples, that topical applications of glycerol and propylene glycol, two common biocompatible and osmotically active solutions, onto the surface of rat tissue could significantly improve its OCT imaging contrast and depth capability.

© 2001 Optical Society of America

OCIS Codes
(120.5710) Instrumentation, measurement, and metrology : Refraction
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6930) Medical optics and biotechnology : Tissue

Ruikang K. Wang, Xiangqun Xu, Valery V. Tuchin, and James B. Elder, "Concurrent enhancement of imaging depth and contrast for optical coherence tomography by hyperosmotic agents," J. Opt. Soc. Am. B 18, 948-953 (2001)

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1180 (1991). [CrossRef] [PubMed]
  2. C. A. Puliafito, M. R. Hee, C. P. Lin, E. Reichel, J. S. Schuman, J. S. Duker, J. A. Izatt, E. A. Swanson, and J. G. Fujimoto, “Imaging of macular disease with optical coherence tomography,” Ophthalmology 102, 217–229 (1995). [CrossRef] [PubMed]
  3. K. Kobayashi, J. A. Izzat, M. D. Kulkarni, J. Willis, and M. V. Sivak, “High resolution cross sectional imaging of the gastrointestinal tract using optical coherence tomography: preliminary results,” Gastrointest. Endosc. 47, 515–523 (1998). [CrossRef] [PubMed]
  4. R. K. Wang, J. B. Elder, and V. Smith, “High resolution optical tomographic imaging of human gastrointestinal tissue in vitro with optical coherence tomography,” Proc. SPIE 4224, 294–298 (2000). [CrossRef]
  5. C. Pitris, C. Jesser, S. A. Boppart, D. Stamper, M. E. Brezinski, and J. G. Fujimoto, “Feasibility of optical coherence tomography for high resolution imaging of human gastrointestinal tract malignancies,” J. Gastroenterol. 35, 87–92 (2000). [CrossRef]
  6. B. W. Colston, M. J. Everett, L. B. Da Silva, L. L. Otis, P. Stroeve, and H. Nathel, “Imaging of hard- and soft-tissue structure in the oral cavity by optical coherence tomography,” Appl. Opt. 37, 3582–3585 (1998). [CrossRef]
  7. J. Welzel, E. Lankenau, R. Birngruber, and R. Engelhardt, “Optical coherence tomography of the human skin,” J. Am. Acad. Dermatol. 37, 958–963 (1997). [CrossRef]
  8. J. M. Schmitt, M. Yadlowsky, and R. F. Bonner, “Subsurface imaging of living skin with optical coherence tomography,” Dermatology. 191, 93–98 (1995). [CrossRef]
  9. J. G. Fujimoto, B. Bouma, G. J. Tearney, S. A. Boppart, C. Pitris, J. F. Southern, and M. E. Brezinski, “New technology for high speed and high resolution optical coherence tomography,” Ann. N.Y. Acad. Sci. 838, 95–107 (1998). [CrossRef]
  10. R. K. Wang, “Modeling optical properties of soft tissue by fractal distribution of scatters,” J. Mod. Opt. 47, 103–120 (2000). [CrossRef]
  11. V. V. Tuchin, Tissue Optics, Tutorial Texts in Optical Engineering, Vol. TT38 (SPIE Press, Bellingham, WA, 2000).
  12. J. M. Schmitt, A. Knuttel, M. Yadlowsky, and M. A. Eckhaus, “Optical-coherence tomography of a dense tissue: statistics of attenuation and backscattering,” Phys. Med. Biol. 39, 1705–1720 (1994). [CrossRef] [PubMed]
  13. V. V. Tuchin, I. L. Maksimova, D. A. Zimnyakov, I. L. Kon, A. K. Mavlutov, and A. A. Mishin, “Light propagation tissues with controlled optical properties,” J. Biomed. Opt. 2, 401–417 (1997). [CrossRef] [PubMed]
  14. V. V. Bakutkin, I. L. Maksimova, T. N. Semyonova, V. V. Tuchin, and I. L. Kon, “Controlling of optical properties of sclera,” Proc. SPIE 2393, 137–141 (1995). [CrossRef]
  15. D. A. Zimnyakov, V. V. Tuchin, A. A. Mishin, I. L. Kon, A. N. Serov, “In-vivo human sclera structure analysis using tissue optical immersion effect,” Proc. SPIE 2673, 233–242 (1996). [CrossRef]
  16. G. Vargas, E. K. Chan, J. K. Barton, H. G. Grady III, and A. J. Welch, “Use of an agent to reduce scattering in skin,” Lasers Surg. Med. 24, 133–141 (1999). [CrossRef] [PubMed]
  17. E. Lankenau, J. Welzel, R. Bringruber, and R. Engelhardt, “In vivo tissue measurements with optical low coherence tomography,” Proc. SPIE 2981, 29–36 (1996).
  18. T. R. Porter and F. Xie, “Transient myocardial contrast after initial exposure to diagnostic ultrasound pressures with minute doses of intravenously injected microbubbles: demonstration and potential mechanisms,” Circulation 92, 2391–2395 (1995). [CrossRef] [PubMed]
  19. S. R. Gregory, “Physical properties of glycerine,” in Glycerine: A Key Cosmetic Ingredient, E. Jungermann and N. O. V. Sonntag, eds. (Marcel Dekker, New York, 1991).
  20. “Product description of propylene glycol,” retrieved August 2000, http://www.fluidscience.com.
  21. “Fact sheets of ethylene glycol and propylene glycol,” Agency for Toxic Substances and Disease Registry, retrieved August 2000, http://www.atsdr.cdc.gov.
  22. G. F. Odland, “Structure of the skin,” in Physiology, Biochemistry, and Molecular Biology of the Skin, L. A. Goldsmith, ed. (Oxford University Press, New York, 1991), pp. 3–62.
  23. B. R. Nelson, D. J. Fader, M. Gillard, G. Majmudar, and T. M. Johnson, “Pilot histologic and ultrastructural-study of the effects of medium-depth chemical facial peels on dermal collagen in patients with actinically damaged skin,” J. Am. Acad. of Dermatol. 32, 472–478 (1995). [CrossRef]
  24. I. F. Cilesiz and A. J. Welch, “Light dosimetry: effects of dehydration and thermal damage on the optical properties of the human aorta,” Appl. Opt. 32, 477–487 (1993). [CrossRef] [PubMed]

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