Fluorescence correlation spectroscopy when fluorescent nanoemitters are diffusing in the vicinity of a dielectric mirror is studied both theoretically and experimentally. We demonstrate that two important effects occur when the confocal detection volume is located on the mirror’s surface. First, the count rate per emitter is significantly enhanced owing to control of spontaneous emission and enhancement of the excitation field. Second, interference fringes in the excitation beam give rise to a new characteristic time in the photocurrent’s autocorrelation function. This new time is found to be independent of the transverse excitation field’s beam waist and permits accurate measurement of diffusion coefficients without any a priori knowledge of the confocal volume geometry.

© 2003 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• Theoretical study of detection of a dipole emitter through an objective with high numerical aperture (OL)
• Confocal microscopy with a refractive microlens-pinhole array (OL)
• Investigation of a Double-Pass Confocal Scanning Microscope with a Self-Pumped Phase-Conjugate Mirror (AO)
• Omnidirectional Mirror Design With Quarter-Wave Dielectric Stacks (AO)
• Loss Factors of Mirrors for a gravitational wave Antenna (AO)

Related Conference Papers

• Retinal Motion Tracking in Adaptive Optics Scanning Laser Ophthalmoscopy
• Retinal Motion Tracking in Adaptive Optics Scanning Laser Ophthalmoscopy
• Simple Confocal Microscopy Based on Single Hollow-Core Photonic Bandgap Fiber
• Damage and Distortion Measurement for HR, Low-Loss Mirrors to 100+ MW/cm2 CW Laser Intensity
• Damage and Distortion Measurement for HR, Low-Loss Mirrors to 100+ MW/cm2 CW Laser Intensity