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Journal of the Optical Society of America B

Journal of the Optical Society of America B


  • Vol. 21, Iss. 6 — Jun. 1, 2004
  • pp: 1210–1215

Single-molecule orientations determined by direct emission pattern imaging

M. Andreas Lieb, James M. Zavislan, and Lukas Novotny  »View Author Affiliations

JOSA B, Vol. 21, Issue 6, pp. 1210-1215 (2004)

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We demonstrate a new method of determining the three-dimensional dipole orientations of single molecules by direct imaging of the emission patterns in the back focal plane of a high-numerical-aperture objective lens. We compare the reconstructed emission-dipole orientations with a previously established method of absorption-dipole mapping. We find that, for a given number of emitted photons, emission pattern imaging provides better accuracy (1°–2°) than absorption-dipole mapping of single molecules. Compared with some other methods for emission-dipole mapping, the presented method is (1) less sensitive to optical aberrations and adjustment and (2) data analysis is simplified because radiation patterns can be expressed in a simple analytical form.

© 2004 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(260.2510) Physical optics : Fluorescence
(300.2140) Spectroscopy : Emission
(300.2530) Spectroscopy : Fluorescence, laser-induced

M. Andreas Lieb, James M. Zavislan, and Lukas Novotny, "Single-molecule orientations determined by direct emission pattern imaging," J. Opt. Soc. Am. B 21, 1210-1215 (2004)

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