Femtosecond pulse characterization and compensation using multiphoton intrapulse interference phase scan (MIIPS) [Opt. Lett.29, 775 (2004)] was rigorously tested. MIIPS was found to have 3 mrad precision within the 90 nm bandwidth of the pulses. Group-velocity dispersion measurements of glass and quartz provided independent accuracy tests. Phase distortions from high-numerical-aperture objectives were measured and corrected using MIIPS, an important requirement for reproducible two-photon microscopy. Phase compensation greatly improved the pulse-shaping results through a more accurate delivery of continuous and binary phase functions to the sample. MIIPS measurements were possible through the scattering of biological tissue, a consideration for biomedical imaging.
© 2006 Optical Society of America
Original Manuscript: May 6, 2005
Revised Manuscript: August 12, 2005
Manuscript Accepted: August 17, 2005
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics
Bingwei Xu, Jess M. Gunn, Johanna M. Dela Cruz, Vadim V. Lozovoy, and Marcos Dantus, "Quantitative investigation of the multiphoton intrapulse interference phase scan method for simultaneous phase measurement and compensation of femtosecond laser pulses," J. Opt. Soc. Am. B 23, 750-759 (2006)