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Journal of the Optical Society of America B

Journal of the Optical Society of America B


  • Vol. 18, Iss. 11 — Nov. 1, 2001
  • pp: 1695–1700

Simulation of confocal microscopy through scattering media with and without time gating

Marcus Magnor, Peter Dorn, and Wolfgang Rudolph  »View Author Affiliations

JOSA B, Vol. 18, Issue 11, pp. 1695-1700 (2001)

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An efficient and fast simulation technique is presented to calculate characteristic features of confocal imaging through scattering media. The simulation can predict the time-resolved confocal response to pulsed illumination that allows optimizing of imaging contrast when time-gating techniques are applied. Modest computational effort is sufficient to obtain contrast predictions for arbitrary numerical aperture, focus depth, pinhole size, and scattering density, while the simulation accuracy is independent of scattering density and pinhole size. In the case of isotropic scattering, our results indicate that reflection-mode confocal imaging through scattering media is limited to μd3.5 optical thicknesses for continuous-wave illumination. If time-gating is applied, imaging through scattering densities of μd8 is theoretically possible.

© 2001 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(290.4210) Scattering : Multiple scattering
(290.7050) Scattering : Turbid media
(320.7100) Ultrafast optics : Ultrafast measurements

Marcus Magnor, Peter Dorn, and Wolfgang Rudolph, "Simulation of confocal microscopy through scattering media with and without time gating," J. Opt. Soc. Am. B 18, 1695-1700 (2001)

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  1. J. G. Fujimoto, S. de Silvestri, and E. P. Ippen, “Femtosecond optical ranging in biological tissue,” Opt. Lett. 11, 150–152 (1986). [CrossRef]
  2. J. M. Schmitt, “Optical coherence tomography (OCT): a review,” IEEE J. Sel. Top. Quantum Electron. 5, 1205–1215 (1999). [CrossRef]
  3. K. M. Yoo, Feng Liu, and R. R. Alfano, “Biological materials probed by the temporal and angular profiles of the backscattered ultrafast laser pulses,” J. Opt. Soc. Am. B 7, 1685–1693 (1990). [CrossRef]
  4. R. Vreeker, M. P. Van Albada, R. Sprik, and A. Lagendijk, “Femtosecond time-resolved measurements of weak localization of light,” Phys. Lett. A 132, 51–54 (1988). [CrossRef]
  5. E. Baiger, C. Hauger, and W. Zinth, “Imaging within highly scattering media using time-resolved backscattering of femtosecond pulses,” Appl. Phys. B 67, 257–261 (1998). [CrossRef]
  6. M. Kempe, A. Z. Genack, W. Rudolph, and P. Dorn, “Ballistic and diffuse light detection in confocal and heterodyne imaging systems,” J. Opt. Soc. Am. A 14, 216–223 (1997). [CrossRef]
  7. M. S. Patterson, B. Chance, and B. C. Wilson, “Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties,” Appl. Opt. 28, 2331–2336 (1989). [CrossRef] [PubMed]
  8. S. T. Flock, M. S. Patterson, B. C. Wilson, and D. R. Wyman, “Monte Carlo modeling of light propagation in highly scattering tissue I: Model predictions and comparison with diffusion theory,” IEEE Trans. Biomed. Eng. 36, 1162–1167 (1989). [CrossRef] [PubMed]
  9. E. Tinet, S. Avrillier, and J. M. Tualle, “Fast semianalytical Monte Carlo simulation for time-resolved light propagation in turbid media,” J. Opt. Soc. Am. A 13, 1903–1915 (1996). [CrossRef]
  10. J. M. Schmitt, A. Knüttel, and M. Yadlowsky, “Confocal microscopy in turbid media,” J. Opt. Soc. Am. A 11, 2226–2235 (1994). [CrossRef]
  11. J. M. Schmitt and K. Ben-Letaief, “Efficient Monte Carlo simulation of confocal microscopy in biological tissue,” J. Opt. Soc. Am. A 13, 952–961 (1996). [CrossRef]
  12. C. M. Blanca and C. Saloma, “Efficient analysis of temporal broadening of a pulsed focused Gaussian beam in scattering media,” Appl. Opt. 38, 5433–5437 (1999). [CrossRef]
  13. T. Wilson, ed., Confocal Microscopy (Academic, San Diego Calif., 1990), p. 11.
  14. H. C. Van De Hulst, Multiple Light Scattering: Tables, Formulas, and Applications (Academic, San Diego, Calif., 1980), Vol. 1, p. 9.
  15. M. Magnor, “Reflection mode confocal microscopy through scattering media,” M.S. thesis (University of New Mexico, Albuquerque, New Mexico, 1997), p. 63.
  16. J. M. Schmitt and A. Knüttel, “Model of optical coherence tomography of heterogenous tissue,” J. Opt. Soc. Am. A 14, 1231–1242 (1997). [CrossRef]
  17. M. R. Hee, J. A. Izatt, J. M. Jacobson, and J. G. Fujimoto, “Femtosecond transillumination optical coherence tomography,” Opt. Lett. 18, 950–952 (1993). [CrossRef] [PubMed]
  18. A. Schmidt, R. Corey, and P. Saulnier, “Imaging through random media by use of low-coherence optical heterodyning,” Opt. Lett. 20, 404–406 (1995). [CrossRef] [PubMed]
  19. M. Kempe, W. Rudolph, and E. Welsch, “Comparative study of confocal and heterodyne microscopy for imaging through scattering media,” J. Opt. Soc. Am. A 13, 46–52 (1996). [CrossRef]

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