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Journal of the Optical Society of America B

Journal of the Optical Society of America B

| OPTICAL PHYSICS

  • Vol. 21, Iss. 8 — Aug. 1, 2004
  • pp: 1486–1493

Autoconfocal microscopy with nonlinear transmitted light detection

Thomas Pons and Jerome Mertz  »View Author Affiliations


JOSA B, Vol. 21, Issue 8, pp. 1486-1493 (2004)
http://dx.doi.org/10.1364/JOSAB.21.001486


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Abstract

We describe a simple and robust technique for transmission confocal laser scanning microscopy wherein the detection pinhole is replaced by a thin second-harmonic generation crystal. The advantage of this technique is that self-aligned confocality is achieved without the need for signal descanning. We derive the point-spread function of our instrument and quantify both signal degradation and background rejection when imaging deep within a turbid slab. As an example, we consider a slab whose index of refraction fluctuations exhibit Gaussian statistics. Our model is corroborated by experiment.

© 2004 Optical Society of America

OCIS Codes
(030.6600) Coherence and statistical optics : Statistical optics
(110.0180) Imaging systems : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

Citation
Thomas Pons and Jerome Mertz, "Autoconfocal microscopy with nonlinear transmitted light detection," J. Opt. Soc. Am. B 21, 1486-1493 (2004)
http://www.opticsinfobase.org/josab/abstract.cfm?URI=josab-21-8-1486

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