OSA's Digital Library

Journal of the Optical Society of America B

Journal of the Optical Society of America B


  • Vol. 21, Iss. 8 — Aug. 1, 2004
  • pp: 1486–1493

Autoconfocal microscopy with nonlinear transmitted light detection

Thomas Pons and Jerome Mertz  »View Author Affiliations

JOSA B, Vol. 21, Issue 8, pp. 1486-1493 (2004)

View Full Text Article

Enhanced HTML    Acrobat PDF (386 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We describe a simple and robust technique for transmission confocal laser scanning microscopy wherein the detection pinhole is replaced by a thin second-harmonic generation crystal. The advantage of this technique is that self-aligned confocality is achieved without the need for signal descanning. We derive the point-spread function of our instrument and quantify both signal degradation and background rejection when imaging deep within a turbid slab. As an example, we consider a slab whose index of refraction fluctuations exhibit Gaussian statistics. Our model is corroborated by experiment.

© 2004 Optical Society of America

OCIS Codes
(030.6600) Coherence and statistical optics : Statistical optics
(110.0180) Imaging systems : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

Thomas Pons and Jerome Mertz, "Autoconfocal microscopy with nonlinear transmitted light detection," J. Opt. Soc. Am. B 21, 1486-1493 (2004)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. C. Yang and J. Mertz, “Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection,” Opt. Lett. 28, 224–227 (2003). [CrossRef] [PubMed]
  2. V. I. Tatarski, Wave Propagation in a Turbulent Medium (Dover, New York, 1967).
  3. S. M. Rytov, Y. A. Kravtsov, and V. I. Tatarskii, Principles of Statistical Radiophysics (Springer-Verlag, Heidelberg, 1987).
  4. J. W. Goodman, Statistical Optics (Wiley, New York, 1984).
  5. A. Ishimaru, Wave Propagation and Scattering in Random Media, IEEE/OUP Series on Electromagnetic Wave Theory (Institute of Electrical and Electronics Engineers, Piscataway, N.J., 1978).
  6. T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, New York, 1984).
  7. C. J. R. Sheppard and X. Q. Mao, “Three-dimensional imaging in a microscope,” J. Opt. Soc. Am. A 6, 1260–1269 (1989). [CrossRef]
  8. J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill New York, 1968).
  9. N. Streibl, “Depth transfer by an imaging system,” Opt. Acta 31, 1233–1241 (1984). [CrossRef]
  10. M. Born and E. Wolf, Principles of Optics (Academic, New York, 1970).
  11. V. Tuchin, Tissue Optics (SPIE Press, Bellingham, Wash. 2000).
  12. E. Beaurepaire and J. Mertz, “Epifluorescence collection in two-photon microscopy,” Appl. Opt. 41, 5376–5382 (2002). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited