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Journal of the Optical Society of America B

Journal of the Optical Society of America B


  • Editor: G. I. Stegeman
  • Vol. 23, Iss. 11 — Nov. 1, 2006
  • pp: 2418–2424

Measuring the dynamics of circular dichroism in a pump–probe experiment with a Babinet–Soleil Compensator

Claire Niezborala and François Hache  »View Author Affiliations

JOSA B, Vol. 23, Issue 11, pp. 2418-2424 (2006)

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Circular dichroism contains rich information on the conformation of molecules and, in particular, of biomolecules, and measuring its variation in a pump–probe experiment is very promising but also very challenging. We propose a new technique to measure pump-induced variation of the circular dichroism, which is based on the measurement of the probe ellipticity and its variation with the pump. This technique has the advantage that it does not require modulation of the probe polarization, which allows modulation of the pump intensity. We show theoretically and demonstrate experimentally that this technique is very sensitive and user friendly. We also show that it can be used to measure pump-induced change in the optical rotation, allowing for a complete characterization of pump-induced optical activity.

© 2006 Optical Society of America

OCIS Codes
(260.2130) Physical optics : Ellipsometry and polarimetry
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Ultrafast Optics

Original Manuscript: May 26, 2006
Revised Manuscript: July 26, 2006
Manuscript Accepted: August 4, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Claire Niezborala and François Hache, "Measuring the dynamics of circular dichroism in a pump-probe experiment with a Babinet-Soleil Compensator," J. Opt. Soc. Am. B 23, 2418-2424 (2006)

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  1. J. W. Lewis, R. F. Tilton, C. M. Einterz, S. J. Milder, I. D. Kuntz, and D. S. Kliger, "New technique for measuring circular dichroism changes on a nanosecond time scale. Application to (carbonmonoxy)myoglobin and (carbonmonoxy)hemoglobin," J. Phys. Chem. 89, 289-294 (1985). [CrossRef]
  2. X. Xie and J. D. Simon, "Picosecond time-resolved circular dichroism study of protein relaxation in myoglobin following photodissociation of CO," J. Am. Chem. Soc. 112, 7802-7803 (1990). [CrossRef]
  3. T. Dartigalongue and F. Hache, "Observation of sub-100ps conformational changes in photolyzed carbonmonoxy-myoglobin probed by time-resolved circular dichroism," Chem. Phys. Lett. 415, 313-316 (2005). [CrossRef]
  4. T. Dartigalongue and F. Hache, "Classical calculation of myoglobin circular dichroism spectrum: simulation of a time-resolved experiment," J. Chem. Phys. 123, 184901 (2005). [CrossRef] [PubMed]
  5. S. C. Bjorling, R. A. Golbeck, S. J. Milder, C. A. Randall, J. W. Lewis, and D. S. Kliger, "Analysis of optical artifacts in ellipsometric measurements of time-resolved circular dichroism," J. Phys. Chem. 95, 4685-4694 (1991). [CrossRef]
  6. T. Dartigalongue and F. Hache, "Precise alignment of a longitudinal Pockels cell for time-resolved circular dichroism experiments," J. Opt. Soc. Am. B 20, 1780-1787 (2003). [CrossRef]
  7. N. Sreerama and R. W. Woody, "Circular dichroism of peptides and proteins," in Circular Dichroism. Principles and Applications, 2nd ed., N.Berova, K.Nakanishi, and R.W.Woody, eds. (Wiley-VCH, 2000), pp. 601-620.
  8. C. Brosseau, Fundamentals of Polarized Light (Wiley, 1998).
  9. X. Xie and J. D. Simon, "Picosecond circular dichroism spectroscopy: a Jones matrix analysis," J. Opt. Soc. Am. B 7, 1673-1684 (1990). [CrossRef]
  10. H. Graener, G. Seifert, and A. Laubereau, "Direct observation of rotational relaxation times by time-resolved infrared spectroscopy," Chem. Phys. Lett. 172, 435-439 (1990). [CrossRef]
  11. J. W. Petrich, C. Poyart, and J. L. Martin, "Photophysics and reactivity of heme proteins: a femtosecond absorption study of hemoglobin, myoglobin and protoheme," Biochemistry 27, 4049-4060 (1988). [CrossRef] [PubMed]
  12. S. Franzen, L. Kiger, C. Poyart, and J.-L. Martin, "Heme photolysis occurs by ultrafast excited state metal-to-ring charge transfer," Biophys. J. 80, 2372-2385 (2001). [CrossRef] [PubMed]
  13. D. W. Urry, "The heme chromophore in the ultraviolet," J. Biol. Chem. 242, 4441-4448 (1967). [PubMed]

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