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Journal of the Optical Society of America B

Journal of the Optical Society of America B

| OPTICAL PHYSICS

  • Editor: Henry M. Van Driel
  • Vol. 25, Iss. 11 — Nov. 1, 2008
  • pp: 1907–1913

Phase-controlled polarization coherent anti-Stokes Raman scattering microscopy for high-sensitivity and high-contrast molecular imaging

Fake Lu, Wei Zheng, and Zhiwei Huang  »View Author Affiliations


JOSA B, Vol. 25, Issue 11, pp. 1907-1913 (2008)
http://dx.doi.org/10.1364/JOSAB.25.001907


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Abstract

We report on a phase-controlled polarization coherent anti-Stokes Raman scattering (CARS) microscopy for high-sensitivity and high-contrast molecule vibrational imaging. By changing the phase difference between the two CARS signals (i.e., a weak resonant CARS and a strong nonresonant CARS signal) simultaneously generated from the same focal volume of the sample, the complete constructive (in-phase) and destructive (out-of-phase) interference CARS images can be acquired for image processing. The directly digital subtraction between the constructive and destructive interference CARS images yields a fivefold improvement in signal-to-background ratios compared with conventional CARS while providing an approximately 20-fold amplification of a resonant CARS signal compared with conventional polarization CARS imaging. We demonstrate this technique by imaging 1 μ m polystyrene beads and unstained human epithelial cells in aqueous environments.

© 2008 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.3970) Nonlinear optics : Microparticle nonlinear optics
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(100.3175) Image processing : Interferometric imaging

ToC Category:
Nonlinear Optics

History
Original Manuscript: June 17, 2008
Manuscript Accepted: September 15, 2008
Published: October 28, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Fake Lu, Wei Zheng, and Zhiwei Huang, "Phase-controlled polarization coherent anti-Stokes Raman scattering microscopy for high-sensitivity and high-contrast molecular imaging," J. Opt. Soc. Am. B 25, 1907-1913 (2008)
http://www.opticsinfobase.org/josab/abstract.cfm?URI=josab-25-11-1907


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