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Journal of the Optical Society of America B

Journal of the Optical Society of America B


  • Editor: Grover Swartzlander
  • Vol. 31, Iss. 9 — Sep. 1, 2014
  • pp: 2208–2213

Influence of nonlinear effects on the three-photon excitation of L-Tryptophan in water using phase-shaped pulses

Alexander Patas, Georg Achazi, Christopher Winta, and Albrecht Lindinger  »View Author Affiliations

JOSA B, Vol. 31, Issue 9, pp. 2208-2213 (2014)

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We show that nonlinear effects like self-phase modulation in a sample have to be considered for phase control of three-photon excitations. Furthermore, we demonstrate the control of three-photon excitation of L-Tryptophan in water using a pulse-shaping setup. Simulations of the propagation of the laser pulses in the cuvette exhibit good agreement with the experimental fluorescence scans at different laser intensities and show large discrepancies when neglecting nonlinear effects prior to the three-photon process. This can lead to improvements in selective excitation of amino acids by a near-infrared femtosecond laser source.

© 2014 Optical Society of America

OCIS Codes
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(320.5540) Ultrafast optics : Pulse shaping

ToC Category:

Original Manuscript: April 1, 2014
Revised Manuscript: June 19, 2014
Manuscript Accepted: June 22, 2014
Published: August 26, 2014

Virtual Issues
Vol. 9, Iss. 11 Virtual Journal for Biomedical Optics

Alexander Patas, Georg Achazi, Christopher Winta, and Albrecht Lindinger, "Influence of nonlinear effects on the three-photon excitation of L-Tryptophan in water using phase-shaped pulses," J. Opt. Soc. Am. B 31, 2208-2213 (2014)

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  1. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990). [CrossRef]
  2. S. Hell and E. H. K. Stelzer, “Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation,” Opt. Commun. 93, 277–282 (1992). [CrossRef]
  3. R. Heintzmann and G. Ficz, “Breaking the resolution limit in light microscopy,” Brief. Funct. Genomic. Proteomic. 5, 289–301 (2006).
  4. I. Pastirk, J. D. Cruz, K. Walowicz, V. Lozovoy, and M. Dantus, “Selective two-photon microscopy with shaped femtosecond pulses,” Opt. Express 11, 1695–1701 (2003). [CrossRef]
  5. J. P. Ogilvie, D. Débarre, X. Solinas, J. Martin, E. Beaurepaire, and M. Joffre, “Use of coherent control for selective two-photon fluorescence microscopy in live organisms,” Opt. Express 14, 759–766 (2006). [CrossRef]
  6. J. D. Cruz, V. Lozovoy, and M. Dantus, “Coherent control improves biomedical imaging with ultrashort shaped pulses,” J. Photochem. Photobiol. A Chem. 180, 307–313 (2006).
  7. R. Pillai, C. Boudoux, G. Labroille, N. Olivier, I. Veilleux, E. Farge, M. Joffre, and E. Beaurepaire, “Multiplexed two-photon microscopy of dynamic biological samples with shaped broadband pulses,” Opt. Express 17, 12741–12752 (2009). [CrossRef]
  8. D. Kobat, N. Horton, and C. Xu, “In vivo two-photon microscopy to 1.6-mm depth in mouse cortex,” J. Biomed. Opt. 16, 106014 (2011). [CrossRef]
  9. N. Horton, K. Wang, D. Kobat, C. Clark, F. Wise, C. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7, 205–209 (2013). [CrossRef]
  10. C. Xu, W. Zipfel, J. B. Shear, R. Williams, and W. Webb, “Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy,” Proc. Natl. Acad. Sci. USA 93, 10763–10768 (1996).
  11. S. Schenkl, F. van Mourik, N. Friedman, M. Sheves, R. Schlesinger, S. Haacke, and M. Chergui, “Insights into excited-state and isomerization dynamics of bacteriorhodopsin from ultrafast transient UV absorption,” Proc. Natl. Acad. Sci. USA 103, 4101–4106 (2006).
  12. D. Sharma, J. Léonard, and S. Haacke, “Ultrafast excited-state dynamics of tryptophan in water observed by transient absorption spectroscopy,” Chem. Phys. Lett. 489, 99–102 (2010). [CrossRef]
  13. J. Chen, H. Kawano, Y. Nabekawa, H. Mizuno, A. Miyawaki, T. Tanabe, F. Kannari, and K. Midorikawa, “Selective excitation between two-photon and three-photon fluorescence with engineered cost functions,” Opt. Express 12, 3408–3414 (2004). [CrossRef]
  14. V. Lozovoy, I. Pastirk, K. Walowicz, and M. Dantus, “Multiphoton intrapulse interference. II. Control of two- and three-photon laser induced fluorescence with shaped pulses,” J. Chem. Phys. 118, 3187–3196 (2003). [CrossRef]
  15. T. Wu, J. Tang, B. Hajj, and M. Cui, “Phase resolved interferometric spectral modulation (prism) for ultrafast pulse measurement and compression,” Opt. Express 19, 12961–12968 (2011). [CrossRef]
  16. G. Agrawal, Nonlinear Fiber Optics (Springer, 2000).
  17. Newport, “The effect of dispersion on ultrashort pulses,” 2014, http://www.newport.com/the-effect-of-dispersion-on-ultrashort-pulses/602091/1033/content.aspx .
  18. A. Couairon, L. Sudrie, M. Franco, B. Prade, and A. Mysyrowicz, “Filamentation and damage in fused silica induced by tightly focused femtosecond laser pulses,” Phys. Rev. B 71, 125435 (2005).
  19. P. Devi, V. Lozovoy, and M. Dantus, “Measurement of group velocity dispersion of solvents using 2-cycle femtosecond pulses: experiment and theory,” AIP Adv. 1, 032166 (2011).
  20. Z. Wilkes, S. Varma, Y. Chen, H. Milchberg, T. Jones, and A. Ting, “Direct measurements of the nonlinear index of refraction of water at 815 and 407  nm using single-shot supercontinuum spectral interferometry,” Appl. Phys. Lett. 94, 211102 (2009). [CrossRef]
  21. R. Boyd, Nonlinear Optics (Academic, 2003).
  22. D. Meshulach and Y. Silberberg, “Coherent quantum control of multiphoton transitions by shaped ultrashort optical pulses,” Phys. Rev. A 60, 1287–1292 (1999). [CrossRef]
  23. A. Patas, G. Achazi, N. Hermes, M. Pawłowska, and A. Lindinger, “Contrast optimization of two-photon processes after a microstructured hollow-core fiber demonstrated for dye molecules,” Appl. Phys. B 112, 579–586 (2013). [CrossRef]
  24. G. Achazi, N. Hermes, A. Patas, D. Tolksdorf, and A. Lindinger, “Polarization-shaped laser pulses for improved fluorescence anisotropy contrast,” Eur. Phys. J. D 67, 1–5 (2013). [CrossRef]

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