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Journal of the Optical Society of America B

Journal of the Optical Society of America B

| OPTICAL PHYSICS

  • Vol. 4, Iss. 3 — Mar. 1, 1987
  • pp: 337–350

Fluorescence emission at dielectric and metal-film interfaces

Edward H. Hellen and Daniel Axelrod  »View Author Affiliations


JOSA B, Vol. 4, Issue 3, pp. 337-350 (1987)
http://dx.doi.org/10.1364/JOSAB.4.000337


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Abstract

It is well known that the classical optical properties of a bare or metal-film-coated dielectric surface significantly the emission pattern of a fluorophore in close proximity to it. Most previous classical calculations of this perturb model the fluorophore as a continuous fixed-amplitude dipole acting as a simple radiator. However, for effect modeling steady-state excitation, a fixed-power dipole is more appropriate. This modification corresponds to normalizing fixed-amplitude dipole intensities by the total dissipated power, which is itself dependent on fluorophore orientation and proximity to the surface. The results for the fixed-power model differ nontrivially from the fixed-amplitude model. Using the fixed-power dipole model, we calculate the observation-angle-dependent intensity as a function of the fluorophore’s orientation and distance from the surface. The surface can have an intermediate layer of arbitrary thickness on it, which is used to model a metal-film-coated dielectric. In addition, general expressions are derived for the emission power as observed through a circular-aperture collection system (such as a microscope objective) located on either side of the interface. These expressions are applied to several common cases of fluorophore spatial and orientational distributions at bare glass–water and metal-film-coated glass-water interfaces. The results suggest practical experimental approaches for measuring the spatial and orientational distribution of fluorophores adsorbed at a surface, utilizing the distance-dependent fluorescence near a metalized surface and optimizing the collection efficiency from a well-defined volume near a quenching surface.

© 1987 Optical Society of America

History
Original Manuscript: August 14, 1986
Manuscript Accepted: November 25, 1986
Published: March 1, 1987

Citation
Edward H. Hellen and Daniel Axelrod, "Fluorescence emission at dielectric and metal-film interfaces," J. Opt. Soc. Am. B 4, 337-350 (1987)
http://www.opticsinfobase.org/josab/abstract.cfm?URI=josab-4-3-337

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