OSA's Digital Library

Journal of the Optical Society of Korea

Journal of the Optical Society of Korea

| PUBLISHED BY THE OPTICAL SOCIETY OF KOREA

  • Vol. 7, Iss. 4 — Dec. 25, 2003
  • pp: 240–244

Reagentless Determination of Human Serum Components Using Infrared Absorption Spectroscopy

Sang-Joon Hahn, Gil-Won Yoon, Gun-Shik Kim, and Seung-Han Park  »View Author Affiliations


Journal of the Optical Society of Korea, Vol. 7, Issue 4, pp. 240-244 (2003)


View Full Text Article

Acrobat PDF (363 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Simultaneous determination of concentrations for four major components in human blood serum was investigated using a Fourier-transform mid-infrared spectroscopy. Infrared spectra of human blood serum were measured in 8.404 ∼ 10.25 <TEX>${\mu}m$</TEX> range where the highest absorption peaks of glucose are located. A partial least square (PLS) algorithm was utilized to establish a calibration model for determining total protein, albumin, globulin and glucose levels which are commonly measured metabolites. The standard error of cross validation obtained from our multivariate calibration model was 0.24 g/dL for total protein, 0.15 g/dL for albumin, 0.17 g/dL for globulin, and 6.68 mg/dL for glucose, which are comparable with or meet the criteria for clinical use. The results indicate that the infrared absorption spectroscopy can be used to predict the concentrations of clinically important metabolites without going through a chemical process with a reagent.

© 2003 Optical Society of Korea

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(190.0190) Nonlinear optics : Nonlinear optics
(280.0280) Remote sensing and sensors : Remote sensing and sensors

History
Original Manuscript: October 7, 2003
Published: December 1, 2003

Citation
Sang-Joon Hahn, Gil-Won Yoon, Gun-Shik Kim, and Seung-Han Park, "Reagentless Determination of Human Serum Components Using Infrared Absorption Spectroscopy," J. Opt. Soc. Korea 7, 240-244 (2003)
http://www.opticsinfobase.org/josk/abstract.cfm?URI=josk-7-4-240


Sort:  Year  |  Journal  |  Reset

References

  1. G. Budinova, J. Salva, and K. Volka, "Application of Molecular Spectroscopy in the Mid-Infrared Region to the Determination of Glucose and Cholesterol in Whole Blood and in Blood Serum," Appl. Spectrosc., vol. 51, no. 5, pp. 631-639, 1997 [CrossRef]
  2. G. Janatsch, and J. D. Kruse-Jarres, "Multivariate calibration for assays in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma," Anal. Chem., vol. 61, p. 2016, 1989 [CrossRef]
  3. F. Cadet, C. Robert, and B. Offmann, "Simultaneous Determination of Sugars by Multivariate Analysis Applied to Mid-Infrared Spectra of Biological Samples," Appl. Spectrosc., vol. 51, no. 3, pp. 369-375, 1997 [CrossRef]
  4. R Vonach, J. Buschmann, R. Falkowski, R. Schindler, B. Lendl, and R. Kellner, "Application of MidInfrared Transmission Spectrometry to the Direct Determination of Glucose in Whole Blood," Appl. Specirosc., vol. 51, no. 6, pp. 820-822, 1998 [CrossRef]
  5. H. M. Heise and R.Marbach, "Human oral mucosa studies with varying blood glucose concentration by non-invasive ATR-FT-IR-Spectroscopy," Cellular and Molecular Biology, vol. 44, no. 6, pp. 899-912, 1998
  6. N. A. Cingo, G. W. Small, and M. A. Arnold, "Determination of glucose in a synthetic biological matrix with decimated time-domain filtered near-infrared interferogram data," Vib. Spec., vol. 23, pp. 103-117, 2000 [CrossRef]
  7. I. Amato, "In Search of Human Touch," Science, vol. 258, no. 5085, pp. 892-895, 1992 [CrossRef]
  8. M. A. Arnold, G. W. Small, "Determination of physiological levels of glucose in an aqueous matrix with digitally filtered Fourier transform near-infrared spectra," Anal. Chem., vol. 62, no. 14, pp. 1457-1464 (1990) [CrossRef]
  9. R. A. Shaw and H. H. Mantsch, "Multianalyte Serum Assays from Mid-IR Spectra of Dry Films on Glass Slides," Appl. Specirosc., vol. 54, no. 6, pp. 885-889, 2000 [CrossRef]
  10. H. Martens and T. Naes, Multivariate Calibration (John Wiley and Sons, New York, 1989), pp. 1-30
  11. R. Kramer, Chemometric Techniques for Quantitative Analysis (Marcel Dekker, Inc., New York, 1998) pp.1-8
  12. H. M. Heise, Infrared and Raman Spectroscopy of Biological Materials, U-H Gremlich and B. Yan Eds., (Marcel Dekker, New York, 2001), pp. 259-322
  13. O. S. Khalil, "Spectroscopic and Clinical Aspects of Noninvasive Glucose Measurements," Clin. Chem., vol. 45, no. 2, pp. 165-177, 1999
  14. S. Sasic and Y. Ozaki, "Statistical Two-Dimensional Correlation Spectroscopy: Its Theory and Applications to Sets of Vibrational Spectra," Anal. Chem., vol. 73, no. 10, pp. 2294-2301, 2001 [CrossRef]
  15. C. A. Burtis and E. R. Ashwood, Tietz Textbook of Clinical Chemistry (3rd edition, W.B. Saunders Co., 1999) pp. 375-377

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited