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Journal of the Optical Society of Korea

Journal of the Optical Society of Korea

| PUBLISHED BY THE OPTICAL SOCIETY OF KOREA

  • Vol. 12, Iss. 2 — Jun. 25, 2008
  • pp: 107–111

Single-molecule Detection of Fluorescence Resonance Energy Transfer Using Confocal Microscopy

Sung-Hyun Kim, Don-Seong Choi, and Do-Seok Kim  »View Author Affiliations


Journal of the Optical Society of Korea, Vol. 12, Issue 2, pp. 107-111 (2008)


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Abstract

We demonstrated single-molecule fluorescence resonance energy transfer (FRET) from single donor-acceptor dye pair attached to a DNA with a setup based on a confocal microscope. Singlestrand DNAs were immobilized on a glass surface with suitable inter-dye distance. Energy transfer efficiency between the donor and the acceptor dyes attached to the DNA was measured with different lengths of DNA. Photobleaching of single dye molecule was observed and used as a sign of single-molecule detection. We could achieve high enough signal-to-noise ratio to detect the fluorescence from a single-molecule, which allows real-time observation of the distance change between single dye pairs in nanometer scale.

© 2008 Optical Society of Korea

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.0180) Microscopy : Microscopy
(300.0300) Spectroscopy : Spectroscopy

History
Original Manuscript: February 29, 2008
Revised Manuscript: May 21, 2008
Manuscript Accepted: May 23, 2008
Published: June 25, 2008

Citation
Sung-Hyun Kim, Don-Seong Choi, and Do-Seok Kim, "Single-molecule Detection of Fluorescence Resonance Energy Transfer Using Confocal Microscopy," J. Opt. Soc. Korea 12, 107-111 (2008)
http://www.opticsinfobase.org/josk/abstract.cfm?URI=josk-12-2-107


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