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Journal of the Optical Society of Korea

Journal of the Optical Society of Korea


  • Vol. 13, Iss. 3 — Sep. 25, 2009
  • pp: 335–340

Ultrashort Pulsed Laser Machining for Biomolecule Trapping

Hae-Woon Choi, Dave F. Farson, L.James Lee, and Ho Lee  »View Author Affiliations

Journal of the Optical Society of Korea, Vol. 13, Issue 3, pp. 335-340 (2009)

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Ultrashort pulse laser drilling of polycarbonate track-etched membrane (pTEM) material was used to fabricate a mouse embryo cell trapping device. Holes with a diameter of <TEX>$2{\mu}m$</TEX> to <TEX>$5{\mu}m$</TEX> were fabricated on a <TEX>$10{\mu}m$</TEX> thick membrane using a femtosecond laser with a 150 fs pulse width and 775 nm wavelength and multiple-pulse irradiation. In cell trapping tests, the overall cell occupancy of the machined holes in the fabricated pTEM was found to be more than 80%. The results of a single pulse and multiple pulse irradiation were compared in terms of the surface quality. It was generally found that a single pulse with high energy was less desirable than irradiation with multiple pulses of lower energy.

© 2009 Optical Society of Korea

OCIS Codes
(140.0140) Lasers and laser optics : Lasers and laser optics
(170.7160) Medical optics and biotechnology : Ultrafast technology

Original Manuscript: June 26, 2009
Revised Manuscript: September 10, 2009
Manuscript Accepted: September 14, 2009
Published: September 25, 2009

Hae-Woon Choi, Dave F. Farson, L.James Lee, and Ho Lee, "Ultrashort Pulsed Laser Machining for Biomolecule Trapping," J. Opt. Soc. Korea 13, 335-340 (2009)

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