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Journal of the Optical Society of Korea

Journal of the Optical Society of Korea


  • Vol. 13, Iss. 3 — Sep. 25, 2009
  • pp: 392–397

Detection of Avian Influenza-DNA Hybridization Using Wavelength-scanning Surface Plasmon Resonance Biosensor

Shin-Ae Kim, Sung-June Kim, Sang-Hun Lee, Tai-Hyun Park, Kyung-Min Byun, Sung-Guk Kim, and Michael L. Shuler  »View Author Affiliations

Journal of the Optical Society of Korea, Vol. 13, Issue 3, pp. 392-397 (2009)

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We designed a wavelength interrogation-based surface plasmon resonance (SPR) biosensor to detect avian influenza DNA (AI-DNA). Hybridization reactions between target AI-DNA probes and capture probes immobilized on a gold surface were monitored quantitatively by measuring the resonance wavelength in the visible waveband. The experimental results were consistent with numerical calculations. Although the SPR detection technique does not require the DNA to be labeled, we also evaluated fluorescently-labeled targets to verify the hybridization behavior of the AI-DNA. Changes in resonance were found to be linearly proportional to the amount of bound analyte. A wavelength interrogation-type SPR biosensor can be used for rapid measurement and high-throughput detection of highly pathogenic AI viruses.

© 2009 Optical Society of Korea

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(240.6680) Optics at surfaces : Surface plasmons
(310.0310) Thin films : Thin films

Original Manuscript: June 1, 2009
Revised Manuscript: July 3, 2009
Manuscript Accepted: August 5, 2009
Published: September 25, 2009

Shin-Ae Kim, Sung-June Kim, Sang-Hun Lee, Tai-Hyun Park, Kyung-Min Byun, Sung-Guk Kim, and Michael L. Shuler, "Detection of Avian Influenza-DNA Hybridization Using Wavelength-scanning Surface Plasmon Resonance Biosensor," J. Opt. Soc. Korea 13, 392-397 (2009)

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  1. K. Hidari, S. Shimada, Y. Suzuki, and T. Suzuki, "Binding kinetics of influenza viruses to sialic acid-containing carbohydrates," Glycoconj. J. 24, 583-590 (2007) [CrossRef]
  2. J. Xu, D. Suarez, and D. S. Gottfried, "Detection of avian influenza virus using an interferometric biosensor," Anal. Bioanal. Chem. 389, 1193-1199 (2007) [CrossRef]
  3. A. J. Thiel, A. G. Frutos, C. E. Jordan, R. M. Corn, and L. M. Smith, "In situ surface plasmon resonance imaging detection of DNA hybridization to oligonucleotide arrays on gold surfaces," Anal. Chem. 69, 4948-4956 (1997)
  4. J. Homola, S. S. Yee, and G. Gauglitz, "Surface plasmon resonance sensors: review," Sens. Actuators B 54, 3-15 (1999) [CrossRef]
  5. M. G. Manera, J. Spadavecchia, A. Leone, F. Quaranta, R. Rella, D. Dell"arri, M. Minunni, M. Mascini, and P.Siciliano, "Surface plasmon resonance imaging technique for nucleic acid detection," Sens. Actuators B 130, 82-87 (2008) [CrossRef]
  6. W. Y. Chen, W. P. Hu, Y. D. Su, A. Taylor, S. Jiang, and G. L. Chang, "A multisport DNA chip fabricated with mixed ssDNA/oligo(ethylene glycol) self-assembled monolayers for detecting the effect of secondary structures on hybridization by SPR imaging," Sens. Actuators B 125, 607-614 (2007) [CrossRef]
  7. K. M. Byun, M. L. Shuler, S. J. Kim, S. J. Yoon, and D. Kim, "Sensitivity enhancement of surface plasmon resonance imaging using periodic metallic nanowires," J. Lightwave Technol. 26, 1472-1478 (2008) [CrossRef]
  8. M. Mir and I. Katakis, "Target label-free, reagentless electrochemical DNA biosensor based on sub-optimum displacement," Talanta 75, 432-441 (2008) [CrossRef]
  9. A. B. Steel, T. M. Herne, and M. J. Tarlov, "Electrochemical quantitation of DNA immobilized on gold," Anal. Chem. 70, 4670-4677 (1998) [CrossRef]
  10. K. M. Byun, S. J. Yoon, D. Kim, and S. J. Kim, "Experimental study of sensitivity enhancement in surface"plasmon resonance biosensors by use of periodic metallic nanowires," Opt. Lett. 32, 1902-1904 (2007) [CrossRef]
  11. M. G. Moharam and T. K. Gaylord, "Diffraction analysis dielectric surface-relief gratings," J. Opt. Soc. Am. 72, 1385-1392 (1982) [CrossRef]
  12. J. Cesario, R. Quidant, G. Badenes, and S. Enoch, "Electromagnetic coupling between a metal nanoparticle grating and a metallic surface," Opt. Lett. 30, 3404-3406 (2005) [CrossRef]
  13. Y. Kanamori, K. Hane, H. Sai, and H. Yugami, "100 nm period silicon antireflection structures fabricated using a porous alumina membrane mask," Appl. Phys. Lett. 78, 142-143 (2001) [CrossRef]
  14. E. D. Palik, Handbook of Optical Constants of Solids(Academic Press, Orlando, FL, USA, 1985)

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