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Journal of the Optical Society of Korea

Journal of the Optical Society of Korea


  • Vol. 15, Iss. 1 — Mar. 1, 2011
  • pp: 61–67

Visualization of Epidermis and Dermal Cells in ex vivo Human Skin Using the Confocal and Two-photon Microscopy

Sang-Hoon Choi, Wi-Han Kim, Yong-Joong Lee, Ho Lee, Weon-Ju Lee, Jung-Dug Yang, Jong-Won Shim, and Jin-Woong Kim  »View Author Affiliations

Journal of the Optical Society of Korea, Vol. 15, Issue 1, pp. 61-67 (2011)

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The confocal laser scanning microscopy and two-photon microscopy was implemented based on a single laser source and an objective lens. We imaged and compared the morphology of identical sites of ex vivo human skin using both microscopes. The back-scattering emission from the sample provided the contrast for the confocal microscopy. The intrinsic autofluorescence and the second harmonic generation were used as the luminescence source for the two-photon microscopy. The wavelength of the Ti:Sapphire laser was tuned at 710 nm, which corresponds to the excitation peak of NADH and FAD in skin tissue. The various cell layers in the epidermis and the papillary dermis were clearly distinguished by both imaging modalities. The two-photon microscopy more clearly visualized the intercellular region and the nucleus of the cell compared to the confocal microscopy. The fibrous structures in the dermis were more clearly resolved by the confocal microscopy. Numerous cells in papillary dermal layer, as deep as 100 µm, were observed in both CLSM and two-photon microscopy. While most previous studies focused on fibrous structure imaging (collagen and elastin fiber) in the dermis, we demonstrated that the combined imaging with the CLSM and two-photon microscopy can be applied for the non-invasive study of the population, distribution and metabolism of papillary dermal cells in skin.

© 2011 Optical Society of Korea

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(190.4180) Nonlinear optics : Multiphoton processes

Original Manuscript: November 15, 2010
Revised Manuscript: January 19, 2011
Manuscript Accepted: January 19, 2011
Published: March 25, 2011

Sang-Hoon Choi, Wi-Han Kim, Yong-Joong Lee, Ho Lee, Weon-Ju Lee, Jung-Dug Yang, Jong-Won Shim, and Jin-Woong Kim, "Visualization of Epidermis and Dermal Cells in ex vivo Human Skin Using the Confocal and Two-photon Microscopy," J. Opt. Soc. Korea 15, 61-67 (2011)

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