In this study we investigate the use of 2D and 3D scanning optical coherence tomography (OCT) technology for use in apple blotch diagnosis. In order to test the possible application of OCT as a detection tool for apple trees affected by Marssonina coronaria, we conducted several experiments and compared the results from both healthy and infected leaves. Using OCT, we found several distinctive features in the subsurface boundary regions of both the diseased and healthy leaves. Our results indicate that leaves from diseased trees, while still appearing healthy, can be affected by M. coronaria. The A-scan analysis method confirmed that the boundaries found under the subsurface layers can be faint. This shows that M. coronaria can exert its influence on entire apple trees (as opposed to only on leaves with lesions) once it infects healthy trees. Our results indicate that OCT can be used as a noninvasive tool for the diagnosis of fungal disease in apple trees. Microscopic imaging results, performed as a histological study for comparison, correlated well with the OCT results.
© 2012 Optical Society of Korea
(110.4500) Imaging systems : Optical coherence tomography
(120.4630) Instrumentation, measurement, and metrology : Optical inspection
(170.1610) Medical optics and biotechnology : Clinical applications
Original Manuscript: February 17, 2012
Revised Manuscript: April 12, 2012
Manuscript Accepted: April 17, 2012
Published: June 25, 2012
Chang-Ho Lee, Seung-Yeol Lee, Hee-Young Jung, and Jee-Hyun Kim, "The Application of Optical Coherence Tomography in the Diagnosis of Marssonina Blotch in Apple Leaves," J. Opt. Soc. Korea 16, 133-140 (2012)
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