We report a method for optical monitoring of tumors in an animal model using optical coherence tomography (OCT). In a spectral domain OCT system, a superluminescent diode light source with a full width of 66 nm at half maximum and peak wavelength of 950 nm was used to take images having an axial resolution of 6.8 μm. Cancer cells of PC-3 were cultured and inoculated into the hypodermis of auricle tissues in BALB/c nude mice. We observed tumor formation and growth at the injection region of cancer cells in vivo and obtained the images of tumor mass center and sparse circumferences. On the 5th day from an inoculation of cancer cells, histological images of the tumor region using cross-sectional slicing and dye staining of specimens were taken in order to confirm the correlation with the high resolution OCT images. The OCT image of tumor mass compared with normal tissues was analyzed using its A-scan data so as to obtain a tissue attenuation rate which increases according to tumor growth.
© 2013 Optical Society of Korea
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization
Original Manuscript: October 22, 2012
Revised Manuscript: January 16, 2013
Manuscript Accepted: January 18, 2013
Published: February 25, 2013
Hyun-Woo Song, Sang-Won Lee, Myung-Hwan Jung, Kye Ryung Kim, Seungkyoung Yang, Jeong Won Park, Min-Sook Jeong, Moon Youn Jung, and Seunghwan Kim, "Optical Monitoring of Tumors in BALB/c Nude Mice Using Optical Coherence Tomography," J. Opt. Soc. Korea 17, 91-96 (2013)
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