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Journal of Optical Technology

Journal of Optical Technology

| SIMULTANEOUS RUSSIAN-ENGLISH PUBLICATION

  • Vol. 80, Iss. 11 — Nov. 1, 2013
  • pp: 655–660

Study of the action of broad-band terahertz radiation on the functional activity of cells

M. V. Duka (Tsurkan), Yu. S. Nesgovorova, O. A. Smolyanskaya, V. G. Bespalov, I. V. Kudryavtsev, A. V. Polevshchikov, M. K. Serebryakova, I. V. Nazarova, and A. S. Trulev  »View Author Affiliations


Journal of Optical Technology, Vol. 80, Issue 11, pp. 655-660 (2013)
http://dx.doi.org/10.1364/JOT.80.000655


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Abstract

In order to determine the limits of the biologically safe maximum allowable levels of pulsed terahertz (THz) radiation, a prototype of apparatus has been developed for the irradiation of cell cultures immersed in a liquid medium with broad-band THz radiation in the 0.05–1.2 THz frequency range. The action of THz radiation on the functional activity of the cells of a number of biological cultures, including mouse thymocytes and splenocytes, has been investigated. This was evaluated by means of flow-through cytometry, using fluorescence-intensity analysis of DNA-binding stains. It is shown that THz radiation with a mean power density of up to 10  μW/cm2 and a time of action of 1 min has no effect on the variation of the functional activity of the cells. No statistically reliable variations were detected in the relationship of living cells and cells at different stages of apoptosis or in the distribution of cells over the phases of the cellular cycle. It is concluded that THz radiation with such characteristics can be used for medical spectroscopy.

© 2014 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(320.7160) Ultrafast optics : Ultrafast technology

History
Original Manuscript: July 30, 2013
Published: January 23, 2014

Citation
M. V. Duka (Tsurkan), Yu. S. Nesgovorova, O. A. Smolyanskaya, V. G. Bespalov, I. V. Kudryavtsev, A. V. Polevshchikov, M. K. Serebryakova, I. V. Nazarova, and A. S. Trulev, "Study of the action of broad-band terahertz radiation on the functional activity of cells," J. Opt. Technol. 80, 655-660 (2013)
http://www.opticsinfobase.org/jot/abstract.cfm?URI=jot-80-11-655


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