Abstract

This paper discusses the problem of obtaining reliable quantitative experimental data when the functional activity of brain cells is studied by fluorescence microscopy, using specific calcium dyes. A method is developed for determining the calcium-ion concentration from the current fluorescence intensity at a single wavelength, starting from the coloration protocol and the excitation conditions and making it unnecessary to carry out calibration in each experiment.

© 2011 OSA

Ion concentration quantification with the help of single-wavelength dye fluorescence

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